Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60422
DC FieldValueLanguage
dc.contributor.authorChen, S.Y.en_US
dc.contributor.author賴建成zh_TW
dc.contributor.authorLi, K.I.en_US
dc.contributor.authorYu, C.S.en_US
dc.contributor.authorWang, J.S.en_US
dc.contributor.authorHu, Y.C.en_US
dc.contributor.authorLai, C.C.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-09T05:56:28Z-
dc.date.available2014-06-09T05:56:28Z-
dc.identifier.issn0003-2700zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/60422-
dc.description.abstractAlthough matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) analysis is an important tool for analyzing and characterizing biomolecules of varying complexity, the sensitivity of MALDI-TOFMS is dependent on proper preparation of the sample, a process that is oftentimes problematic and requires considerable expertise. In this study, we have developed a radiate microstructure chip on which samples can be concentrated for analysis by MALDI-TOFMS. The sample/matrix mixture was deposited onto the central space of the well on the chip and allowed to dry. Microscopic analysis confirmed that the applied samples were confined to the central zone. Sample spots focused on the chip were much smaller than those on an unmodified plate with the same total volume. Optimizing processes of several preparation factors were also performed to ensure matrix homogeneity in our chip. Analysis of the samples with MALDI-TOFMS showed that the signals from samples on our chip were significantly greater than those on the unmodified plate. The feasibility of using this chip to detect peptides and phosphopeptides was also demonstrated.en_US
dc.language.isoen_USzh_TW
dc.relationAnalytical Chemistryen_US
dc.relation.ispartofseriesAnalytical Chemistry, Volume 82, Issue 14, Page(s) 5951-5957.en_US
dc.relation.urihttp://dx.doi.org/10.1021/ac101426nen_US
dc.subjectionization mass-spectrometryen_US
dc.subjectlaser-desorption ionizationen_US
dc.subjectporousen_US
dc.subjectsiliconen_US
dc.subjecttof-msen_US
dc.subjectproteinsen_US
dc.subjectsensitivityen_US
dc.subjectpeptidesen_US
dc.subjectdesorption/ionizationen_US
dc.subjectfabricationen_US
dc.subjecttargetsen_US
dc.titleA Radiate Microstructure MALDI Chip for Sample Concentration and Detectionen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1021/ac101426nzh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
Appears in Collections:分子生物學研究所
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