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標題: Proteomic analysis of chondrocytes exposed to pressure
作者: Juang, Y.M.
Lee, C.Y.
Hsu, W.Y.
Lin, C.T.
Lai, C.C.
Tsai, F.J.
關鍵字: mass spectrometry;chondrocytes;proteomic;2D electrophoresis;Pressure;articular-cartilage;protein families;cell-surface;matrix;expression;reveals;stress;subfamilies;panther;ions
Project: Biomedical Chromatography
期刊/報告no:: Biomedical Chromatography, Volume 24, Issue 12, Page(s) 1273-1282.
Chondrocytes are the only cell type present in mature articular cartilage (2-5% of total tissue). The biological activities of the chondrocyte population are regulated by genetic, biologic and biochemical factors, as well as environmental factors (stress, flow and electric field). Although compressive forces within joint articular cartilage are required for maintenance of the normal composition of articular cartilage, there is a lack of knowledge about the number of pressure-related proteins expressed in articular cartilage. Two-dimensional gel electrophoresis (2-DE) and high-performance liquid chromatography-electrospray/tandem mass spectrometry (HPLC/ESI-MS/MS) were used to identify the levels of pressure-related proteins expressed by chondrocytes grown in the presence or absence of hydrostatic pressure. A total of 266 spots were excised from the gels and analyzed by HPLC/ESI-MS/MS. Functional classification of up-regulated proteins indicated that energy and protein fate were the main biological processes occurring in pressurized chondrocytes. Furthermore, membrane-bound transferrin-like protein p97, a marker of chondrocyte differentiation, was only expressed in chondrocytes under hydrostatic pressure. These data suggest that hydrostatic pressure can induce cell differentiation by increasing the expression level of energy metabolism-and protein fate-related proteins, indicating that hydrostatic pressure may be needed for normal biosynthesis and differentiation of articular chondrocytes. Copyright (C) 2010 John Wiley & Sons, Ltd.
ISSN: 0269-3879
DOI: 10.1002/bmc.1436
Appears in Collections:分子生物學研究所

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