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標題: Identification of over-expressed proteins in oral squamous cell carcinoma (OSCC) patients by clinical proteomic analysis
作者: Lo, W.Y.
Tsai, M.H.
Tsai, Y.
Hua, C.H.
Tsai, F.J.
Huang, S.Y.
Tsai, C.H.
Lai, C.C.
關鍵字: oral squamous cell carcinoma (OSCC);oral cancer;proteomics;LC-MS/MS;real-time quantitative RT-PCR;heat-shock proteins;tumor-associated proteins;superoxide-dismutase;mass-spectrometry;prognostic-significance;oxidative stress;gene-expression;brain-tumors;cancer;carcinogenesis
Project: Clinica Chimica Acta
期刊/報告no:: Clinica Chimica Acta, Volume 376, Issue 1-2, Page(s) 101-107.
Background: Oral cancer is a worldwide problem. It is a universal aggressive disease in the population of smoking and drinking. The oral cancer mortality has been ranked 5th place in Taiwan in male cancer patients. A number of protein markers for oral cancer are still not applicable in large populations. Proteomic technologies provide excellent tools for rapid screening of a large number of potential biomarkers in malignant cells. Method: Proteomics and real-time quantitative RT-PCR were used to analyze over-expressed proteins in 10 OSCC patients. Result: Forty-one proteins were identified as commonly over-expressed in OSCC tissues. In OSCC tissues, alpha beta-crystallin, tropomyosin 2, myosin light chain 1, heat shock protein 27 (HSP27), stratifin, thioredoxin-dependent peroxide reductase, flavin reductase, vimentin, rho GDP-dissociation inhibitor 2 (rho GDI-2), glutathione S-transferase Pi (GST-pi) and superoxide dismutase [Mn] (MnSOD) were significantly over-expressed (an average of 7.2, 6.0, 5.7, 4.3, 3.6, 3.4, 3.0, 3.0, 2.6, 2.5, 2.1-fold, respectively). In real-time quantitative RT-PCR analysis, the gene expressions of alpha beta-crystallin, HSP27 and MnSOD were also increased in the cancer tissues, consistent with proteomic results. Conclusion: The identified proteins in this experiment may be used in future studies of carcinogenesis or as diagnostic markers and therapeutic targets for OSCC. (c) 2006 Elsevier B.V. All rights reserved.
ISSN: 0009-8981
DOI: 10.1016/j.cca.2006.06.030
Appears in Collections:分子生物學研究所

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