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|標題:||Identification of over-expressed proteins in oral squamous cell carcinoma (OSCC) patients by clinical proteomic analysis||作者:||Lo, W.Y.
|關鍵字:||oral squamous cell carcinoma (OSCC);oral cancer;proteomics;LC-MS/MS;real-time quantitative RT-PCR;heat-shock proteins;tumor-associated proteins;superoxide-dismutase;mass-spectrometry;prognostic-significance;oxidative stress;gene-expression;brain-tumors;cancer;carcinogenesis||Project:||Clinica Chimica Acta||期刊/報告no：:||Clinica Chimica Acta, Volume 376, Issue 1-2, Page(s) 101-107.||摘要:||
Background: Oral cancer is a worldwide problem. It is a universal aggressive disease in the population of smoking and drinking. The oral cancer mortality has been ranked 5th place in Taiwan in male cancer patients. A number of protein markers for oral cancer are still not applicable in large populations. Proteomic technologies provide excellent tools for rapid screening of a large number of potential biomarkers in malignant cells. Method: Proteomics and real-time quantitative RT-PCR were used to analyze over-expressed proteins in 10 OSCC patients. Result: Forty-one proteins were identified as commonly over-expressed in OSCC tissues. In OSCC tissues, alpha beta-crystallin, tropomyosin 2, myosin light chain 1, heat shock protein 27 (HSP27), stratifin, thioredoxin-dependent peroxide reductase, flavin reductase, vimentin, rho GDP-dissociation inhibitor 2 (rho GDI-2), glutathione S-transferase Pi (GST-pi) and superoxide dismutase [Mn] (MnSOD) were significantly over-expressed (an average of 7.2, 6.0, 5.7, 4.3, 3.6, 3.4, 3.0, 3.0, 2.6, 2.5, 2.1-fold, respectively). In real-time quantitative RT-PCR analysis, the gene expressions of alpha beta-crystallin, HSP27 and MnSOD were also increased in the cancer tissues, consistent with proteomic results. Conclusion: The identified proteins in this experiment may be used in future studies of carcinogenesis or as diagnostic markers and therapeutic targets for OSCC. (c) 2006 Elsevier B.V. All rights reserved.
|Appears in Collections:||分子生物學研究所|
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