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標題: S100A8 is identified as a biomarker of HPV18-infected oral squamous cell carcinomas by suppression subtraction hybridization, clinical proteomics analysis, and immunohistochemistry staining
作者: Lo, W.Y.
Lai, C.C.
Hua, C.H.
Tsai, M.H.
Huang, S.Y.
Tsai, C.H.
Tsai, F.J.
關鍵字: proteomics;oral squamous cell carcinomas;human papilomaviruses 18;suppression subtraction hybridization;S100 calcium-binding protein A8;human-papillomavirus type-16;calcium-binding proteins;gene-expression;neck-cancer;skin carcinogenesis;head;immortalization;keratinocytes;mass;hpv
Project: Journal of Proteome Research
期刊/報告no:: Journal of Proteome Research, Volume 6, Issue 6, Page(s) 2143-2151.
The purpose of this work is to differentiate between the Human papillomaviruses 18 positive ( HPV18+) and negative (HPV18-) oral squamous cell carcinomas (OSCC) in oral cancer patients with cancer-associated oral habits ( betel quid chewing, cigarette smoking, and alcohol drinking). Both gene and protein expression profiles of HPV18+ and HPV18- OSCC were compared: we then further explored the biological effect of HPV in oral cancer. Suppression subtraction hybridization (SSH), clinical proteomics analysis, and immunohistochemistry ( IHC) staining were carried out in the HPV18+ and HPV18- OSCC groups. HPV typing detection revealed that 11 OSCC tissues from 82 patients were positive for HPV18. The SSH experiment showed that 4 cancer-associated genes were highly transcribed within 11 cDNA libraries of HPV18+ OSCC, including poly( ADP-ribose) polymerase I (PARP1), replication protein A2 (RPA2), S100A8, and S100A2. Clinical proteomics analysis indicated that there was over 10-fold overexpression of Stratifin, F-actin capping protein alpha-1 subunit (CapZ alpha-1), Apolipoprotein A-1 ( ApoA-1), Heat-shock protein 27 ( HSP27), Arginase-1, p16(INK4A), and S100 calcium-binding protein A8 ( S100A8) in HPV18+ OSCC. Interestingly, the results from SSH and protemics analysis showed that S100A8 was overexpressed in HPV18+ OSCC. Moreover, IHC staining demonstrated that S100A8 was up-regulated in HPV18+ OSCC tissues. Our results suggest that S100A8 plays an important role in oral carcinogenesis following HPV18 infection; therefore, S100A8 may be a powerful biomarker of HPV18 as well as a potential therapeutic target for HPV18+ OSCC patients. The study is the first to identify S100A8 as a biomarker in HPV-associated cancer. Furthermore, this is also the first study to discover a biomarker by combining SSH, clinical proteomics, and IHC stain analysis in oral cancer-associated research.
ISSN: 1535-3893
DOI: 10.1021/pr060551+
Appears in Collections:分子生物學研究所

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