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標題: Analysis of urinary nucleosides as helper tumor markers in hepatocellular carcinoma diagnosis
作者: Jeng, L.B.
Lo, W.Y.
Hsu, W.Y.
Lin, W.D.
Lin, C.T.
Lai, C.C.
Tsai, F.J.
關鍵字: performance liquid-chromatography;pattern-recognition analysis;breast-cancer patients;hepatitis-b virus;alpha-fetoprotein;mass-spectrometry;excretion;pseudouridine;population;cirrhosis
Project: Rapid Communications in Mass Spectrometry
期刊/報告no:: Rapid Communications in Mass Spectrometry, Volume 23, Issue 11, Page(s) 1543-1549.
Hepatocellular carcinoma (HCC) is a common neoplasm in Taiwan, for which early diagnosis is difficult and the prognosis is usually poor. HCC is usually diagnosed by abdominal sonography and serum alpha-fetoprotein (AFP) detection. Modified nucleosides, regarded as indicators for the whole-body turnover of RNAs, are excreted in abnormal amounts in the urine of patients with malignancies and can serve as tumor markers. We analyzed the excretion patterns of urinary nucleosides from 25 HCC patients and 20 healthy volunteers by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry (HPLC/ESI-MS/MS) under optimized conditions. The HPLC/ESI-MS/MS approach with selective reaction monitoring (SRM) allowed for the sensitive determination of nucleosides in human urine samples. The mean levels of the urinary nucleosides adenosine, cytidine, and inosine were significantly higher in HCC patients than healthy volunteers (average of 1.78-, 2.26-, and 1.47-fold, respectively). However, the mean levels of urinary 1-methyladenosine, 3-methylcytidine, uridine, and 2'-deoxyguanosine were not significantly different. Combined with the determination of serum AFP levels, the higher levels of urinary adenosine, cytidine, and inosine may be additional diagnosis markers for HCC in Taiwanese patients. Copyright (C) 2009 John Wiley & Sons, Ltd.
ISSN: 0951-4198
DOI: 10.1002/rcm.4034
Appears in Collections:分子生物學研究所

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