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|標題:||Characterization of interleukin-1 beta mRNA expression in chicken macrophages in response to avian reovirus||作者:||Wu, Y.F.
|關鍵字:||double-stranded-rna;reverse transcription-pcr;eimeria-maxima;infection;bursal disease virus;toll-like receptor-3;protein-mu-ns;nf-kappa-b;phylogenetic analysis;mortality syndrome;poult enteritis||Project:||Journal of General Virology||期刊/報告no：:||Journal of General Virology, Volume 89, Page(s) 1059-1068.||摘要:||
Inhibitors of viral disassembly or RNA and protein synthesis, viral disassembly intermediates (infectious subviral particles, ISVP), binary ethylenimine-inactivated virions, and viral particles lacking genomic double-stranded (ds) RNA (empty particles) were used to assess the expression of interleukin-1 beta (IL-1 beta) mRNA in chicken (chIL-1 beta) macrophages in response to avian reovirus. The results demonstrate that two distinct expression patterns of chIL-1 beta mRNA mediated by different steps in viral replication were found. Viral disassembly was required for the induction of a rapid, transient expression pattern of chIL-1 beta mRNA that was rapidly induced at 30 min, with maximal levels reached by 2 h, and fell to a low level within 6 h post-inoculation, while viral RNA synthesis rather than protein translation, which was subsequent to membrane penetration, was required to induce a stable, sustained expression pattern of chIL-1 beta mRNA that occurred at and after 6 h post-inoculation. In addition, the induction of chIL-1 beta mRNA expression by the empty particles and ISVP was extremely weak, compared with the active dsRNA(+) virions or binary ethylenimine-inactivated virions, suggesting that the presence of dsRNA, even if transcriptionally inactive, may be an important factor in this response.
|Appears in Collections:||分子生物學研究所|
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