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標題: Sequence and phylogenetic analysis of interleukin (IL)-1 beta-encoding genes of five avian species and structural and functional homology among these IL-1 beta proteins
作者: Wu, Y.F.
Liu, H.J.
Chiou, S.H.
Lee, L.H.
關鍵字: avian interleukin-1 beta;molecular cloning;protein expression;phylogenetic analysis;homology;reverse transcription-pcr;interferon-gamma;mortality syndrome;poult;enteritis;cloning;virus;macrophages;expression;reovirus;receptor
Project: Veterinary Immunology and Immunopathology
期刊/報告no:: Veterinary Immunology and Immunopathology, Volume 116, Issue 1-2, Page(s) 37-46.
Interleukin (IL)-1 beta-encoding regions of chicken, duck, goose, turkey and pigeon were cloned and sequenced. Each IL-I beta-encoding region of chicken, duck, goose and turkey is 804 nucleotides long and encodes IL-1 beta protein that is 268 amino acids. Pigeon IL-1 beta-encoding region is 810 nucleotides long and encodes IL-1 beta protein that is 270 amino acids. Two one-nucleotide and one four-nucleotide insertions of pigeon IL-1 beta-encoding region sequence were found, resulting in two amino acid insertions in pigeon IL-1 beta. Pairwise sequence analysis showed that the sequence identities of IL-1 beta-encoding genes ranged from 77% to 99%, which were also found for IL-1 beta protein sequence identities, with an average level of both sequence identities of 89%. Phylogenetic analysis indicated that IL-1 beta-encoding regions and the encoded proteins of chicken, duck, goose and turkey clustered together and evolved into a distinct phylogenetic lineage from that of pigeon which evolved into a second lineage. The results from the binding reaction of antiserum against each recombinant IL-1 beta (r IL-1 beta) protein to homologous or heterologous rIL-1 beta, the enhancement levels of K60 mRNA expression in rIL-1 beta-treated DF-1 cells or the reduction levels of K60 mRNA expression in DF-1 cells treated with rIL-1 beta that was preincubated with homologous or heterologous antiserum showed that all five rIL-1 beta were functional active and shared significantly structural and functional homology. (c) 2007 Elsevier B.V. All rights reserved.
ISSN: 0165-2427
DOI: 10.1016/j.vetimm.2006.12.010
Appears in Collections:分子生物學研究所

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