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標題: Novel post-translational modifications of the hemagglutinin and neuraminidase proteins of avian influenza virus expressed by Kluyveromyces lactis
作者: Tsai, S.M.
Chiang, Y.C.
Chin, L.T.
Liu, H.J.
Wang, C.Y.
關鍵字: HA and NA proteins;Avian influenza virus;Kluyveromyces lactis;human airway epithelium;pichia-pastoris;a viruses;glycosylation;yeast;specificity;antibodies;infection;cleavage;receptor
Project: Journal of Virological Methods
期刊/報告no:: Journal of Virological Methods, Volume 175, Issue 2, Page(s) 175-181.
Avian influenza virus (AIV) is an enveloped virus with segmented RNA that belongs to the Orthomyxoviridae. Recently, avian influenza virus isolates have not only posed a significant threat to the poultry industry but also serious public health concerns. The full-length viral hemagglutinin (HA), neuraminidase (NA) or both genes were inserted into the yeast Kluyveromyces lactis genome to allow for secreted expression. Both hemagglutinin and neuraminidase activities were demonstrated for the expressed proteins. Based on PNGase F digestion and immunoassays, N-glycosidically linked high mannose or hybrid-type carbohydrate chains on the HA protein are predominant. It is noteworthy that when co-expression of the HA and NA proteins was carried out, the NA protein was able to react with the HA protein, resulting in deglycosylation in a manner similar to PNGase F digestion. Such post-translational modifications in the HA and NA proteins of AIV are described for the first time. (C) 2011 Elsevier B.V. All rights reserved.
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2011.05.006
Appears in Collections:分子生物學研究所

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