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標題: The Immunomodulatory Activity of Meningococcal Lipoprotein Ag473 Depends on the Conformation Made up of the Lipid and Protein Moieties
作者: Chu, Ching-Liang
Yu, Yen-Ling
Kung, Yueh-Chen
Liao, Pei-Yu
Liu, Ko-Jiunn
Tseng, Yen-Tzu
Lin, Yuan-Chuen
Hsieh, Steve Shih-Yang
Chong, Pele Choi-Sing
Yang, Chiou-Ying
Project: PLoS ONE, Volume 7, Issue 7
We have previously demonstrated that the meningococcal antigen Ag473 in the presence of Freund’s adjuvant can elicit
protective immune responses in mouse challenge model. In this study, we evaluated the structural requirement for the
immunological activity and the possible signaling pathway of recombinant Ag473 antigen produced in E. coli. We found
that lipidated Ag473 (L-Ag473) possesses an intrinsic adjuvant activity that could be attributed to its ability to activate
dendritic cells and promote their maturation. In addition, we found that L-Ag473 can activate human monocytes and
promote maturation of human monocyte-derived dendritic cells. These results provide an indirect support that L-Ag473
may also be immunogenic in human. Interestingly, the observed activity is dependent on the overall conformation of LAg473
because heating and proteinase K treatment can diminish and abolish the activity. Furthermore, our data suggest
a species-differential TLR recognition of L-Ag473. Overall, these data suggest a new paradigm for the ligand-TLR interaction
in addition to demonstrating the self-adjuvanting activity of the vaccine candidate L-Ag473.
DOI: 10.1371/journal.pone.0040873
Appears in Collections:分子生物學研究所

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