Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/60804
DC FieldValueLanguage
dc.contributor.authorChen, Y.T.en_US
dc.contributor.author葉錫東zh_TW
dc.contributor.authorFang, Q.S.en_US
dc.contributor.authorChiang, C.H.en_US
dc.contributor.authorYeh, S.D.en_US
dc.contributor.authorWu, H.W.en_US
dc.contributor.authorYu, T.A.en_US
dc.contributor.author陳玉婷zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-09T06:00:59Z-
dc.date.available2014-06-09T06:00:59Z-
dc.identifier.issn0167-6857zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/60804-
dc.description.abstractLisianthus (Eustoma grandiflorum) is a cut or ornamental flower that is popular all over the world. This ornamental crop, however, lacks an effective weed control method due to its susceptibility to herbicide. In this study, transgenic plants of a lisianthus cultivar were produced using Agrobacterium-mediated delivery of the plasmid pCAMBIA3300, which carried the bialaphos resistance (bar) gene under driven by the CaMV 35S promoter. The transgenic calli were derived from wounded edges of the leaves grown on a shoot regeneration medium containing 100 mg l(-1) cefotaxime and 2 mg l(-1) glufosinate ammonium for 4 weeks. The callus that was detached from the wounded edge of the leaf was transferred to the shoot regeneration medium with 100 mg l(-1) cefotaxime and 5 mg l(-1) glufosinate ammonium for 4 weeks for shoot regeneration. The bar gene integration and expression in the transgenic plants were confirmed by Southern and Northern blot analyses, respectively. Subsequently, the transgenic lines were assessed in vitro and under greenhouse conditions for their resistance to the commercial herbicide Basta(A (R)), which contains glufosinate ammonium as the active component. Six transgenic lines showed high percentages (67-80%) of survival in vitro under the selection condition with glufosinate ammonium (up to 216 mg l(-1)). Under greenhouse conditions, the plants from these six lines remained healthy and exhibited a normal phenotype after spraying with glufosinate ammonium (up to 1,350 mg l(-1)). This is the first paper to provide a detailed survey of transgenic lisianthus expressing the bar gene and exhibiting herbicide-resistance under greenhouse conditions.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Cell Tissue and Organ Cultureen_US
dc.relation.ispartofseriesPlant Cell Tissue and Organ Culture, Volume 102, Issue 3, Page(s) 347-356.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s11240-010-9739-zen_US
dc.subjectAgrobacterium-mediated transformationen_US
dc.subjectHerbicide-resistanceen_US
dc.subjectGreenhouseen_US
dc.subjectevaluationen_US
dc.subjectGlufosinate ammoniumen_US
dc.subjectRootingen_US
dc.subjectagrobacterium-mediated transformationen_US
dc.subjectmicroprojectile bombardmenten_US
dc.subjectlisianthusen_US
dc.subjectculturesen_US
dc.subjectthripsen_US
dc.titleTransgenic Eustoma grandiflorum expressing the bar gene are resistant to the herbicide Basta(A (R))en_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s11240-010-9739-zzh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextno fulltext-
Appears in Collections:基因體暨生物資訊學研究所
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