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標題: Sequence of Closely Related Plasmids Encoding blaNDM-1 in Two Unrelated Klebsiella pneumoniae Isolates in Singapore
作者: Chen, Ying-Tsong
Lin, Ann-Chi
Siu, L.Kristopher
Koh, Tse Hsien
Project: PLoS ONE, Volume 7, Issue 11
Background: Spread of the blaNDM-1 gene that encodes the New Delhi metallo-b-lactamase (NDM-1) in Enterobacteriaceae
is a major global health problem. Plasmids carrying blaNDM-1 from two different multi-drug resistant Klebsiella pneumonia
isolates collected in Singapore were completely sequenced and compared to known plasmids carrying blaNDM-1.
Methodology/Principal Findings: The two plasmids, pTR3 and pTR4, were transferred to Escherichia coli recipient strain J53
and completely sequenced by a shotgun approach using 3-kb paired-end libraries on 454. Although the K. pneumoniae
strains were unrelated by molecular typing using PFGE and MLST, complete sequencing revealed that pTR3 and pTR4 are
identical. The plasmid sequence is similar to the E. coli NDM-1-encoding plasmid p271A, which was isolated in Australia from
a patient returning from Bangladesh. The immediate regions of the blaNDM-1 gene in pTR3/4 are identical to that of p271A,
but the backbone of our plasmid is much more similar to another IncN2 plasmid reported recently, pJIE137, which
contained an additional 5.2-kb CUP (conserved upstream repeat) regulon region in comparison to p271A. A 257-bp element
bounded by imperfect 39-bp inverted repeats (IR) and an incomplete version of this element flanking the 3.6-kb NDM-1-
encoding region were identified in these plasmids and are likely to be the vestiges of an unknown IS.
Conclusions: Although the hosts are not epidemiologically linked, we found that the plasmids bearing the blaNDM-1 gene
are identical. Comparative analyses of the conserved NDM-1-encoding region among different plasmids from K. pneumoniae
and E. coli suggested that the transposable elements and the two unknown IR-associated elements flanking the NDM-1-
encoding region might have aided the spreading of this worrisome resistance determinant.
DOI: 10.1371/journal.pone.0048737
Appears in Collections:基因體暨生物資訊學研究所

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