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標題: Use of Murine Models To Detect the Allergenicity of Genetically Modified Lactococcus lactis NZ9000/pNZPNK
作者: Chiang, Shen-Shih
Liu, Chin-Feng
Ku, Ting-Wei
Mau, Jeng-Leun
Lin, Hsin-Tang
Pan, Tzu-Ming
關鍵字: Genetically modified lactic acid bacteria;Lactobacillus lactis;NZ9000/pNZPNK;pepsin digestion assay;allergenicity assessment;mouse digestive-tract;protein allergenicity;safety assessment;in-vitro;food;nattokinase;mice;expression;survival;strain
Project: Journal of Agricultural and Food Chemistry, Volume 59, Issue 8, Page(s) 3876-3883.
By introducing aprN into Lactococcus lactis NZ9000, the genetically modified L. lactis NZ9000/pNZPNK successfully expressed the nattokinase. The safety assessment of this novel strain was based on allergenicity of pepsin digestion stability and murine model serologic identity. Subjecting to the GM strain and host to pepsin digestion, the soluble fractions and cell debris were fast degraded completely. Feeding with ovalbumin resulted in significantly higher production of IgG1 and IgE as compared to that of L. lactis NZ9000/pNZPNK or L. lactis NZ9000. Further, the serum IgG2a level increased dose-dependently at week 2 and induced immune reaction toward Th1 pathway. Secretion of cytokines IL-4 and IL-10 fed with lactococci was significantly lower than that of the OVA group. L. lactis NZ9000/pNZPNK did not increase the proliferation of type 2 helper T cells in spleen or induce allergenicity in BALB/c mice. On the basis of the results, the new GM lactic acid bacterium is regarded as safe to use.
ISSN: 0021-8561
DOI: 10.1021/jf104656m
Appears in Collections:食品暨應用生物科技學系

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