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標題: Isatis indigotica Induces Hepatocellular Cancer Cell Death via Caspase-Independent Apoptosis-Inducing Factor Translocation Apoptotic Pathway In Vitro and In Vivo
作者: Chung, Ying-Cheng
Tang, Feng-Yao
Liao, Jiunn-Wang
Chung, Chia-Hua
Jong, Ting-Ting
Chen, Shih-Shiung
Tsai, Ching-Hsiu
Chiang, En-Pei
關鍵字: antiproliferation;apoptosis-inducing factor;caspase-independent;apoptotic pathway;cell cycle arrest;tumor growth inhibition;leukemia-cells;chemical-constituents;growth-inhibition;hepatoma-cells;carcinoma;lines;liver;p53;tryptanthrin;indirubin
Project: Integrative Cancer Therapies, Volume 10, Issue 2, Page(s) 201-214.
Isatis indigotica is a biennial herbaceous cruciferous medical herb with antipyretic, antiviral, anti-inflammatory, and anti-endotoxin activity. This study explored the chemotherapeutic potential of I indigotica on human hepatoma cells and investigated the mechanism by which metabolites from I indigotica inhibit hepatoma cell growth. Antitumor activity was discovered in dried I indigotica leaf chloroform extracts (CEDLI). In nude mice xenotransplanted with human hepatoma cells, CEDLI supplementation inhibited tumor growth by similar to 40% compared with nonsupplemented animals without affecting body weight/food intake. CEDLI induced sub-G1 cell cycle arrest and apoptosis in hepatoma cells. Furthermore, CEDLI activates p53 and Bax, reduces Bcl-2 expression, and causes mitochondrial stress and the release of apoptosis-inducing factor into the cytosol followed by its translocation into the nucleus, resulting in hepatoma cell apoptosis. This study provides novel in vivo evidence of I indigotica's antitumor activity. The chemotherapeutic activity against human hepatoma tumorigenesis was because of a distinguished caspase-independent apoptotic pathway.
ISSN: 1534-7354
DOI: 10.1177/1534735410387420
Appears in Collections:食品暨應用生物科技學系

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