Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/61732
標題: Hepatoprotective+Effect+of+Lactic+Acid+Bacteria+in+the+Attenuation+of+Oxidative+Stress+from+tert-Butyl+Hydroperoxide
作者: Ou, Chu-Chyn
Chiu, Yi-Heng
Lin, Shiao-Lin
Chang, Ya-Ju
Huang, Hsin-Yu
Lin, Meei-Yn
關鍵字: lactic acid bacteria;tert-butyl hydroperoxide (t-BHP);oxidative;damage;reactive oxygen species;lipid peroxidation;antioxidant enzymes;alcoholic liver-disease;nonalcoholic steatohepatitis;hepatocellular-carcinoma;glutathione-peroxidase;lipid-peroxidation;r
Project: Journal of Food and Drug Analysis, Volume 20, Issue 1, Page(s) 101-110.
摘要: 
The pathogenesis and progression of liver disease are associated with free radical injury and oxidative stress, which can be partially attenuated by antioxidants and free radical scavengers. Lactic acid bacteria, which have been traditionally used in the production of various fermented foods, are important intestinal microflora and natural antioxidants. The hypothesis that lactic acid bacteria can prevent or decrease tert-butyl hydroperoxide (t-BHP)-induced oxidative damage in HepG2 cells was investigated. Intracellular extracts and heat-killed cells of Lactobacillus acidophilus La12, Lactobacillus delbrueckii ssp. bulgaricus Lb23, Bifidobacterium longum Bl36 and Streptococcus salivarius ssp. thermophilus St28 were used in this study. Lactate dehydrogenase (LDH), alanine aminotransferase (ALT), reactive oxygen species (ROS), thiobarbituric acid reactive substances (TBARS), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRd), glutathione peroxidase (GPx) and glutathione-S-transferase (GST) were determined to explore the influence of lactic acid bacteria intervention on cell damage and antioxidative status. Toxic damage to hepatocytes. by t-BH P was attenuated by lactic acid bacteria (which exerted protective effects by decreasing the risk of accumulated ROS and by reactivating antioxidant enzymes) in HepG2 cells treated with lactic acid bacteria before t-BHP exposure. The results of this study provide new insights into the mechanisms by which lactic acid bacteria with antioxidative properties can help to protect the liver.
URI: http://hdl.handle.net/11455/61732
ISSN: 1021-9498
Appears in Collections:食品暨應用生物科技學系

Show full item record
 

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.