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|標題:||Simultaneous extraction of carotenoids and transfructosylating enzyme from Xanthophyllomyces dendrorhous by a bead beater||作者:||Lee, S.C.
|關鍵字:||Bead beater;Carotenoids;Transfructosylating enzyme;Xanthophyllomyces;dendrorhous;phaffia-rhodozyma;astaxanthin;yeast;trisaccharide;neokestose;mill||Project:||Biotechnology Letters||期刊/報告no：:||Biotechnology Letters, Volume 33, Issue 1, Page(s) 109-112.||摘要:||
Purpose of work Conditions for the cell disruption of Xanthophyllomyces dendrorhous by using a bead beater have been optimized with respect to maximizing the recovery of carotenoids and transfructosylating enzymes in a one-step process. The optimal conditions for a one-step extraction of carotenoids and transfructosylating enzymes from Xanthophyllomyces dendrorhous were: 30 g dry cells/l being disintegrated with 0.5 mm diam. glass beads at 10 degrees C for 15 min using a bead beater. More than 90% yeast disruption and, when extracted with hexane/ethyl acetate, 28.6 mg carotenoids/l in the organic solvent phase and 440 U transfructosylating enzyme activity/mg protein in the water phase were obtained.
|Appears in Collections:||食品暨應用生物科技學系|
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