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標題: Simultaneous extraction of carotenoids and transfructosylating enzyme from Xanthophyllomyces dendrorhous by a bead beater
作者: Lee, S.C.
方 繼
Fang, T.J.
關鍵字: Bead beater;Carotenoids;Transfructosylating enzyme;Xanthophyllomyces;dendrorhous;phaffia-rhodozyma;astaxanthin;yeast;trisaccharide;neokestose;mill
Project: Biotechnology Letters
期刊/報告no:: Biotechnology Letters, Volume 33, Issue 1, Page(s) 109-112.
Purpose of work Conditions for the cell disruption of Xanthophyllomyces dendrorhous by using a bead beater have been optimized with respect to maximizing the recovery of carotenoids and transfructosylating enzymes in a one-step process. The optimal conditions for a one-step extraction of carotenoids and transfructosylating enzymes from Xanthophyllomyces dendrorhous were: 30 g dry cells/l being disintegrated with 0.5 mm diam. glass beads at 10 degrees C for 15 min using a bead beater. More than 90% yeast disruption and, when extracted with hexane/ethyl acetate, 28.6 mg carotenoids/l in the organic solvent phase and 440 U transfructosylating enzyme activity/mg protein in the water phase were obtained.
ISSN: 0141-5492
DOI: 10.1007/s10529-010-0400-z
Appears in Collections:食品暨應用生物科技學系

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