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標題: Preventive effects of guava (Psidium guajava L.) leaves and its active compounds against alpha-dicarbonyl compounds-induced blood coagulation
作者: Hsieh, C.L.
Lin, Y.C.
Yen, G.C.
Chen, H.Y.
關鍵字: alpha-dicarbonyl compounds;methylglyoxal;blood coagulation;guava leaf;extracts;glycation end-products;diabetes-mellitus;antioxidant properties;anticoagulant;thrombin;polysaccharide;antithrombin;aggregation;plasminogen;activation
Project: Food Chemistry
期刊/報告no:: Food Chemistry, Volume 103, Issue 2, Page(s) 528-535.
Diabetes is associated with a hypercoagulable state which may accelerate atherosclerosis, thrombosis and the diabetic microvascular complication. Endogenously produced alpha-dicarbonyl compounds are linked to the pathophysiology of diabetic complications. The effects of alpha-dicarbonyl compounds on coagulation parameters in vitro and the anticoagulant activities of aqueous extracts from guava leaves were examined. Incubation of plasma with glyoxal or methylglyoxal at 0.1 mM showed a significant decrease in thrombin clotting time (TT) (P < 0.05). However, they exhibited slight prolongation of the prothrombin time (PT) at 0.5 mM and no effect on the activated partial thromboplastin time (APTT). In order to define the action mechanism of the hypercoagulant activity, coagulation factors such as fibrinogen and antithrombin III activity were evaluated. The fibrinogen contents in plasma were decreased slightly with increasing concentrations of glyoxal and methylglyoxal. Moreover, methylglyoxal inhibited antithrombin III activity and over 80% of the activity was lost at 1.2 mM methylglyoxal. In contrast, guava leaf extracts exhibited significant inhibition of TT shortening induced by methylglyoxal. Guava leaf extracts and its active phenolic compounds including ferulic acid, gallic acid and quercetin also displayed a protective effect against methylglyoxal-induced loss of activity of antithrombin III. Thus, guava leaf extracts are a potent antiglycative agent and anticoagulant, which can be of great value in the preventive glycation-associated cardiovascular diseases in diabetes. (c) 2006 Elsevier Ltd. All rights reserved.
ISSN: 0308-8146
DOI: 10.1016/j.foodchem.2006.08.022
Appears in Collections:食品暨應用生物科技學系

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