Please use this identifier to cite or link to this item:
標題: FIP-fve Stimulates Interferon-Gamma Production via Modulation of Calcium Release and PKC-alpha Activation
作者: Ou, C.C.
Hsiao, Y.M.
Wu, W.J.
Tasy, G.J.
Ko, J.L.
Lin, M.Y.
關鍵字: FIP;PKC-alpha;IFN-gamma;PBMC;calcium;protein-kinase-c;fungal immunomodulatory protein;blood;mononuclear-cells;nf-kappa-b;intracellular calcium;flammulina-velutipes;t-cells;transcription factors;cellular-responses;edible-mushroom
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 57, Issue 22, Page(s) 11008-11013.
Fungal immunomodulatory protein, FIP-fve, has been isolated from Flammulina velutipes, and its immunomodulatory effects are believed to be associated with the enhanced activation of IFN-gamma-releasing Th1 cells. However, the mechanisms of FIP-fve-mediated signal transduction in the regulation of interferon-gamma (IFN-gamma) gene expression in human peripheral blood mononuclear cells (PBMCs) are still poorly understood. Using fluo-3 AM, we found that FIP-fve induces a rapid elevation in calcium concentration. ELISA, RT-PCR and Western blot assays demonstrated significant increases in the production and mRNA expression of IFN-gamma and protein kinase C-alpha (PKC-alpha) activation in activated PBMCs, which were abolished by EGTA, nifedipine and GO6976. In conclusion, Ca(2+) release and PKC-alpha activation are required for IFN-gamma production induced by FIP-fve in PBMCs.
ISSN: 0021-8561
DOI: 10.1021/jf902725s
Appears in Collections:食品暨應用生物科技學系

Show full item record

Google ScholarTM




Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.