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|標題:||FIP-fve Stimulates Interferon-Gamma Production via Modulation of Calcium Release and PKC-alpha Activation||作者:||Ou, C.C.
|關鍵字:||FIP;PKC-alpha;IFN-gamma;PBMC;calcium;protein-kinase-c;fungal immunomodulatory protein;blood;mononuclear-cells;nf-kappa-b;intracellular calcium;flammulina-velutipes;t-cells;transcription factors;cellular-responses;edible-mushroom||Project:||Journal of Agricultural and Food Chemistry||期刊/報告no：:||Journal of Agricultural and Food Chemistry, Volume 57, Issue 22, Page(s) 11008-11013.||摘要:||
Fungal immunomodulatory protein, FIP-fve, has been isolated from Flammulina velutipes, and its immunomodulatory effects are believed to be associated with the enhanced activation of IFN-gamma-releasing Th1 cells. However, the mechanisms of FIP-fve-mediated signal transduction in the regulation of interferon-gamma (IFN-gamma) gene expression in human peripheral blood mononuclear cells (PBMCs) are still poorly understood. Using fluo-3 AM, we found that FIP-fve induces a rapid elevation in calcium concentration. ELISA, RT-PCR and Western blot assays demonstrated significant increases in the production and mRNA expression of IFN-gamma and protein kinase C-alpha (PKC-alpha) activation in activated PBMCs, which were abolished by EGTA, nifedipine and GO6976. In conclusion, Ca(2+) release and PKC-alpha activation are required for IFN-gamma production induced by FIP-fve in PBMCs.
|Appears in Collections:||食品暨應用生物科技學系|
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