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標題: Hispolon Suppresses SK-Hep1 Human Hepatoma Cell Metastasis by Inhibiting Matrix Metalloproteinase-2/9 and Urokinase-Plasminogen Activator through the PI3K/Akt and ERK Signaling Pathways
作者: Huang, G.J.
Yang, C.M.
Chang, Y.S.
Amagaya, S.
Wang, H.C.
Hou, W.C.
Huang, S.S.
Hu, M.L.
關鍵字: Hispolon;Phellinus linteus;MMP-2;MMP-9;uPA;breast-cancer cells;phellinus-linteus;mmp-9 expression;kappa-b;invasion;angiogenesis;migration;kinase;alpha
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 58, Issue 17, Page(s) 9468-9475.
Cancer metastasis is a primary cause of cancer death. Hispolon is an active phenolic compound of Phellinus linteus, a mushroom that has recently been shown to have antioxidant and anticancer activities. In this study, we first observed that hispolon exerted a dose-dependent inhibitory effect on invasion and motility, but not on adhesion, of the highly metastatic SK-Hep1 cells in the absence of cytotoxicity. Mechanistically, hispolon decreased the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9), and urokinase-plasminogen activator (uPA) in a concentration-dependent manner. Hispolon also inhibited phosphotylation of extracellular signaling-regulating kinase1/2 (ERK1/2), phosphatidylinositol-3-kinase/serine/threonine protein kinase (or protein kinase B (PI3K/Akt), and focal adhesion kinase (FAK). Furthermore, treatment of SK-Hep1 cells with an inhibitor specific for ERK1/2 (PD98256) decreased the expression of MMP-2, and MMP-9. These results demonstrate that hispolon can inhibit the metastasis of SK-Hep1 cells by reduced expression of MMP-2, MMP-9, and uPA through the suppression of the FAK signaling pathway and of the activity of PI3K/Akt and Ras homologue gene family, member A (RhoA). These findings suggest that hispolon may be used as an antimetastatic agent.
ISSN: 0021-8561
DOI: 10.1021/jf101508r
Appears in Collections:食品暨應用生物科技學系

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