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標題: Anti-angiogenic effects and mechanisms of polysaccharides from Antrodia cinnamomea with different molecular weights
作者: Yang, C.M.
Zhou, Y.J.
Wang, R.J.
Hu, M.L.
關鍵字: Angiogenesis;Antrodia cinnamomea;Immunomodulation;Polysaccharides;Vascular endothelial growth factor;reishi polysaccharides;interleukin-12;camphorata;mushrooms;antitumor;cells;extract;tumors
Project: Journal of Ethnopharmacology
期刊/報告no:: Journal of Ethnopharmacology, Volume 123, Issue 3, Page(s) 407-412.
Ethnopharmacological relevance: Antrodia cinnamomea is a popular medicinal mushroom in Taiwan that has been widely used for treatment of various cancers and liver diseases. Aim of the study:This study aimed to investigate the immunomodulatory effect on angiogenesis of polysaccharides from mycelia of Antrodia cinnamomea (PMAC). Materials and methods: PMAC were extracted in boiling water, precipitated with 95% ethanol, and separated into four different molecular weights (<5, 5-30, 30-100, >100 kDa). Tube formation and chorioallantoic membrane (CAM) assay were used to determine the in vitro and ex vivo anti-angiogenic effects. Results: Only the PMAC-mononuclear cells (MNCs)-conditioned medium (CM) with MW > 100 kDa significantly and concentration-dependently decreased the secretion of vascular endothelial growth factor in human leukemia cells and inhibited the matrigel tube formation in human umbilical vein endothelial cells. Similarly only the PMAC-MNC-CM with MW > 100 kDa significantly and concentration-dependently increased the levels of interleukin (IL)-12 and interferon-gamma (IFN-gamma). In addition, the ex vivo CAM assay revealed that only the PMAC with MW > 100 kDa significantly and dose-dependently inhibited neovascularization. Conclusions: PMAC with MW > 100 kDa are anti-angiogenic in vitro and ex vivo, and the effects are likely through immunomodulation. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
ISSN: 0378-8741
DOI: 10.1016/j.jep.2009.03.034
Appears in Collections:食品暨應用生物科技學系

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