Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/62023
DC FieldValueLanguage
dc.contributor.authorYeh, C.M.en_US
dc.contributor.author葉娟美zh_TW
dc.contributor.authorYeh, C.K.en_US
dc.contributor.authorHsu, X.Y.en_US
dc.contributor.authorLuo, Q.M.en_US
dc.contributor.authorLin, M.Y.en_US
dc.date2008zh_TW
dc.date.accessioned2014-06-09T06:26:16Z-
dc.date.available2014-06-09T06:26:16Z-
dc.identifier.issn0099-2240zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/62023-
dc.description.abstractBacillus subtilis and Lactococcus lactis are ideal hosts for the production of extracellular heterologous proteins of major commercial importance. A recombinant gene for the novel Ganoderma lucidium immunomodulatory protein LZ-8, recombinant LZ-8, was designed encoding the same amino acid sequence but using the preferred codons for both strains and was synthesized by overlapping extension PCR. Using the signal peptide (SP) from subtilisin YaB (SPYaB), recombinant LZ-8 was expressed extracellularly in Bacillus subtilis and Lactococcus lactis. In the absence of SPYaB, recombinant LZ-8 was expressed extracellularly in B. subtilis, but not in L. lactis. The three expressed recombinant LZ-8s showed different capacities for modulating the production of Th1 and Th2 cytokines by peripheral blood mononuclear cells and of tumor necrosis factor alpha by a macrophage cell line.en_US
dc.language.isoen_USzh_TW
dc.relationApplied and Environmental Microbiologyen_US
dc.relation.ispartofseriesApplied and Environmental Microbiology, Volume 74, Issue 4, Page(s) 1039-1049.en_US
dc.relation.urihttp://dx.doi.org/10.1128/afm.01547-07en_US
dc.subjectamino-acid-sequenceen_US
dc.subjectling zhi-8 lz-8en_US
dc.subjectgene-expressionen_US
dc.subjectheterologousen_US
dc.subjectproteinen_US
dc.subjectlactobacillus-acidophilusen_US
dc.subjectflammulina velutipesen_US
dc.subjectmolecular-cloningen_US
dc.subjectedible-mushroomen_US
dc.subjectfip-fveen_US
dc.subjectsecretionen_US
dc.titleExtracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactisen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1128/afm.01547-07zh_TW
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
Appears in Collections:食品暨應用生物科技學系
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