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標題: Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis
作者: Yeh, C.M.
Yeh, C.K.
Hsu, X.Y.
Luo, Q.M.
Lin, M.Y.
關鍵字: amino-acid-sequence;ling zhi-8 lz-8;gene-expression;heterologous;protein;lactobacillus-acidophilus;flammulina velutipes;molecular-cloning;edible-mushroom;fip-fve;secretion
Project: Applied and Environmental Microbiology
期刊/報告no:: Applied and Environmental Microbiology, Volume 74, Issue 4, Page(s) 1039-1049.
Bacillus subtilis and Lactococcus lactis are ideal hosts for the production of extracellular heterologous proteins of major commercial importance. A recombinant gene for the novel Ganoderma lucidium immunomodulatory protein LZ-8, recombinant LZ-8, was designed encoding the same amino acid sequence but using the preferred codons for both strains and was synthesized by overlapping extension PCR. Using the signal peptide (SP) from subtilisin YaB (SPYaB), recombinant LZ-8 was expressed extracellularly in Bacillus subtilis and Lactococcus lactis. In the absence of SPYaB, recombinant LZ-8 was expressed extracellularly in B. subtilis, but not in L. lactis. The three expressed recombinant LZ-8s showed different capacities for modulating the production of Th1 and Th2 cytokines by peripheral blood mononuclear cells and of tumor necrosis factor alpha by a macrophage cell line.
ISSN: 0099-2240
DOI: 10.1128/afm.01547-07
Appears in Collections:食品暨應用生物科技學系

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