Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/62024
標題: Chromosomal Integration of a Synthetic Expression Control Sequence Achieves Poly-gamma-glutamate Production in a Bacillus subtilis Strain
作者: Yeh, C.M.
葉娟美
Wang, J.P.
Lo, S.C.
Chan, W.C.
Lin, M.Y.
關鍵字: poly-gamma- glutamate (gamma-PGA);chromosome integration;Bacillus;subtilis;polyglutamic acid production;poly(gamma-glutamic acid);gene;encapsulation;degradation;biopolymer;racemase;system;cells
Project: Biotechnology Progress
期刊/報告no:: Biotechnology Progress, Volume 26, Issue 4, Page(s) 1001-1007.
摘要: 
Poly-gamma-glutamate (gamma-PGA) has applications in food, medical, cosmetic, animal feed, and wastewater industries. Bacillus subtilis DB430, which possesses the gamma-PGA synthesis ywsC-ywtAB genes in its chromosome, cannot produce gamma-PGA. An efficient synthetic expression control sequence (SECS) was introduced into the upstream region of the ywtABC genes, and this resulted in gamma-PGA-producing B. subtilis mutant strains. Mutant B. subtilis PGA6-2 stably produces high levels of gamma-PGA in medium A without supplementation of extra glutamic acid or ammonium chloride. The mutant B. subtilis PGA 6-2 is not only a gamma-PGA producer, but it is also a candidate for the genetic and metabolic engineering of gamma-PGA production. (C) 2010 American Institute of Chemical Engineers Biotechnol. Prog., 26: 1001-1007, 2010
URI: http://hdl.handle.net/11455/62024
ISSN: 8756-7938
DOI: 10.1002/btpr.417
Appears in Collections:食品暨應用生物科技學系

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