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標題: Extracellular Expression of a Recombinant Ganoderma lucidium Immunomodulatory Protein by Food-Grade Lactococcus lactis System
作者: Yeh, C.M.
Yeh, C.K.
Peng, H.J.
Haung, X.H.
Peng, G.Z.
關鍵字: food-grade selection marker;Lactococcus lactis;nisin resistance;rLZ8;nisin resistance determinant;controlled gene-expression;ling zhi-8;lz-8;alpha-galactosidase;positive bacteria;edible-mushroom;delivery-system;cloning vector;acid bacteria;high-level
Project: Food Biotechnology
期刊/報告no:: Food Biotechnology, Volume 23, Issue 2, Page(s) 162-178.
Lactococcus lactis is a food-grade microorganism of major commercial importance in food industry. For commercial application of genetically modified L. lactis, a food-grade expression system is strongly recommended. In this study, two food-grade selection markers, nisin immunity gene nisI and nisin resistance gene nsr, were evaluated as dominant markers for the L. lactis food-grade expression system. By using an efficient PSlpAl promoter fused to a signal peptide from subtilisin YaB (SPYAB), a functional recombinant Ganoderma lucidium immunomodulatory protein rLZ8 was expressed extracellularly in L. lactis. Replacing the antibiotic marker gene into the proper food-grade selection marker nsr gene, the rLZ8 was expressed extracellularly in the food-grade L. lactis system. This study provides a rationale basis for a food-grade system to express functional peptides extracellularly as an important tool for oral administration of genetically modified L. lactis.
ISSN: 0890-5436
DOI: 10.1080/08905430902877313
Appears in Collections:食品暨應用生物科技學系

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