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標題: Green tea catechin inhibits ephrin-A1-mediated cell migration and angiogenesis of human umbilical vein endothelial cells
作者: Tang, F.Y.
Chiang, E.P.I.
Shih, C.J.
關鍵字: epigallocatechin gallate;ephrin-A1;EphA2;PI-3K;ERK-1/2;cell;migration;angiogenesis;receptor tyrosine kinase;eph receptors;cardiovascular development;vascular development;growth-factors;cancer;ligands;phosphorylation;suppression;ephrin-b2
Project: Journal of Nutritional Biochemistry
期刊/報告no:: Journal of Nutritional Biochemistry, Volume 18, Issue 6, Page(s) 391-399.
Angiogenesis, the formation of new blood vessels from preexisting capillaries, is essential for tumor progression and metastasis. During tumor neovascularization, vascular endothelial growth factor and ephrin (Eph) families emerge as critical mediators of angiogenesis. The green tea catechin epigallocatechin gallate (EGCG), a tyrosine kinase inhibitor, has been demonstrated in previous studies to be an effective anti angiogenesis agent. However, the inhibitory effect of green tea catechins on ephrin-A1-mediated tumor angiogenesis has not been demonstrated yet. Thus, in this study, we investigated the molecular mechanism of ephrin-A1-mediated cell migration and angiogenesis, as well as the inhibitory effects of EGCG. Here we show that ephrin-A1 mediates endothelial cell migration and regulates vascular remodeling in tumor neovascularization in vitro. We also demonstrated that ephrin-A1-mediated cell migration required the activation of extracellular-regulated kinase (ERK-1/2) but not of phosphatidylinositol-3-kinase. The green tea catechin EGCG inhibited ephrin-A1-mediated endothelial cell migration, as well as tumor angiogenesis, in a dose-dependent manner. Furthermore, EGCG inhibited the ephrin-A1-mediated phosphorylation of EphA2 and EPK-1/2. Taken together, these data indicated that activation of ERK-1/2 plays an essential role in ephrin-A1-mediated cell migration. EGCG inhibited ephrin-A1-mediated endothelial migration and angiogenesis. It suggests a novel antiangiogenesis application of EGCG in cancer chemoprevention. (C) 2007 Elsevier Inc. All rights reserved.
ISSN: 0955-2863
DOI: 10.1016/j.jnutbio.2006.07.004
Appears in Collections:食品暨應用生物科技學系

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