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標題: Hsian-tsao (Mesona procumbens Heml.) prevents against rat liver fibrosis induced by CCl(4) via inhibition of hepatic stellate cells activation
作者: Shyu, M.H.
Kao, T.C.
Yen, G.C.
關鍵字: Hsian-tsao;Liver fibrogenesis;Oleanolic acid;Ursolic acid;Hepatic;stellate cell;oleanolic acid glycosides;ursolic acid;carbon-tetrachloride;matrix;metalloproteinases;damage;proliferation;constituents;mechanisms;expression;lipocytes
Project: Food and Chemical Toxicology
期刊/報告no:: Food and Chemical Toxicology, Volume 46, Issue 12, Page(s) 3707-3713.
In this study, the protective effect of extract of Hsian-tsao (Mesona procumbens) (EHT) against liver fibrogenesis in carbon tetrachloride (CCl(4))-injured rats was evaluated. The inhibitory effect of oleanolic acid (OA) and Ursolic acid (UA), which are the active compounds in EHT, on the activation of hepatic stellate cells (HSC) was also determined. The results showed that EHT at a dosage of 1.2 g/kg of b.w. significantly reduced the liver injury induced by CCl(4) in rats. It also decreased the activity of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and the deposition of collagen in the liver. Oral administration of EHT reduced the levels of alpha-smooth muscle actin (alpha-SMA) and the activity of metalloproteinases (MMPs) in rats injured by treatment with CCl(4)- In addition, we performed experiments with the rat hepatic stellate cell line HSC-T6 in which we induced the expression of MMP-2 and alpha-SMA with phorbol-12-myristate-13-acetate (PMA). Treating these cells with OA (20 mu M) or UA (10 mu M) caused a decrease in the levels of both proteins. Taken together, our data indicate that EHT can efficiently inhibit CCl(4)-induced liver fibrosis in rats. EHT may therefore be a useful functional food for preventing liver fibrosis. (C) 2008 Elsevier Ltd. All rights reserved.
ISSN: 0278-6915
DOI: 10.1016/j.fct.2008.09.051
Appears in Collections:食品暨應用生物科技學系

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