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|標題:||Antioxidant effects of extracts from Cassia tora L. prepared under different degrees of roasting on the oxidative damage to biomolecules||作者:||Yen, G.C.
|關鍵字:||Cassia tora L.;roasting;antioxidant;prooxidant;oxidative damage;DNA;anthraquinones;dna damage;lipid-peroxidation;prooxidant actions;hydroxyl radicals;hydrogen-peroxide;tea extracts;flavonoids;generation;acid;anthraquinones||Project:||Journal of Agricultural and Food Chemistry||期刊/報告no：:||Journal of Agricultural and Food Chemistry, Volume 47, Issue 4, Page(s) 1326-1332.||摘要:||
The effects of water extracts from Cassia tora L. (WECT) treated with different degrees of roasting (unroasted and roasted at 150, 200, and 250 degrees C) on the oxidative damage to deoxyribose, DNA, and DNA base in vitro were investigated. It was found that WECT alone induced a slight strand breaking of DNA. In the presence of Fe3+/H2O2, WECT accelerated the strand breaking of DNA at a concentration of 2 mu g/mL; however, it decreased with increasing concentrations (>5 mu g/mL) of WECT. WECT also accelerated the oxidation of deoxyribose induced by Fe3+-EDTA/H2O2 at a concentration of 0.2 mg/mL but inhibited the oxidation of deoxyribose induced by Fe3+-EDTA/H2O2/ascorbic acid. Furthermore, WECT accelerated the oxidation of 2'-deoxyguanosine (2'-dG) to form 8-OH-2'-dG induced by Fe3+-EDTA/H2O2. The prooxidant action of WECT on the oxidation of 2'-dG was in the order of unroasted > roasted at 150 degrees C > roasted at 200 degrees C > roasted at 250 degrees C. The decrease in the prooxidant activity of the roasted sample might be due to the reduction in its anthraquinone glycoside content or the formation of antioxidant Maillard reaction products after roasting. Thus, WECT exhibited either a prooxidant or an antioxidant property in the model system that was dependent on the activities of the reducing metal ions, scavenging hydroxyl radical, and chelating ferrous ion.
|Appears in Collections:||食品暨應用生物科技學系|
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