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標題: Identification of an antioxidant, ethyl protocatechuate, in peanut seed testa
作者: Huang, S.C.
Yen, G.C.
Chang, L.W.
Yen, W.J.
Duh, P.D.
關鍵字: peanut seed testa;antioxidant;identification;structure elucidation;liquid chromatography;ethyl protocatechuate;mung bean hulls;methanolic extracts;component
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 51, Issue 8, Page(s) 2380-2383.
The antioxidant activity and identification of the antioxidant component of peanut seed testa were investigated. The antioxidant activity of peanut seed testa was studied in the linoleic acid model system by using the ferric thiocyanate method. Among the five organic solvent extracts, the ethanolic extracts of peanut seed testa (EEPST) produced higher yields and stronger antioxidant activity than other organic solvent extracts. EEPST was separated into 17 fractions on silica gel column chromatography. Fraction 17, which showed the largest yield and significant antioxidant activity, was separated by thin-layer chromatography. Four major antioxidative subfractions were present. Subfraction 17-2 was found to be effective in preventing oxidation of linoleic acid. This subfraction was further fractionated and isolated and characterized by UV, MS, IR, and H-1 NMR techniques. The active compound was identified as ethyl protocatechuate (3,4-dihydroxybenzoic acid ethyl ester).
ISSN: 0021-8561
DOI: 10.1021/jf0210019
Appears in Collections:食品暨應用生物科技學系

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