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標題: Induction of apoptosis by the Lactuca indica L. In human leukemia cell line and its active components
作者: Chen, Y.H.
Chen, H.Y.
Hsu, C.L.
Yen, G.C.
關鍵字: Lactuca indica L.;HL-60 cell;apoptosis;quercetin;n-terminal kinase;nitric-oxide;u937 cells;mitochondria;quercetin;death;tea;activation;flavonoids;glycoside
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 55, Issue 5, Page(s) 1743-1749.
Lactuca indica L. (Compositae family) is used as a folk medicine in anti-inflammatory, antibacterial, antidiabetic, and other medications in Asia. The objectives of this study were to evaluate the antiproliferative effect of ethanol extracts of Lactuca indica L. (EEL) on human leukemic HL-60 cell lines and its active components. The results showed that EEL exhibited strong cytotoxic effects against HL-60 cells; the IC50 value was 313 mu g/mL. Flow cytometric analysis of the externalization of phosphatidylserine (PS) using the annexin V/PI method on EEL-treated HL-60 cells showed a concentration-dependent increase of apoptosis. Moreover, EEL could induce typical DNA fragmentation in a concentration- and time-dependent manner as determined by electrophoresis and TUNEL assays. The treatment of HL-60 cells with EEL induced significant accumulation of cells in the G(0)/G(1) phase, indicating that EEL is a cell-cycle-dependent anticancer agent. Our results also indicate that EEL-induced apoptosis in HL-60 cells is associated with the loss of mitochondrial membrane potential (Delta Psi(m)). EEL contains 5% phenolic compounds, such as quercetin, caffeic acid, rutin, and chlorogenic acid. Among the four active phenolic compounds, quercetin was found to be the most effective in inhibition against cell viability and in alteration of mitochondrial function. Our results suggest that the induction of apoptosis by EEL might offer a pivotal mechanism for its chemopreventive action.
ISSN: 0021-8561
DOI: 10.1021/jf063118t
Appears in Collections:食品暨應用生物科技學系

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