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標題: Naturally occurring flavonoids attenuate high glucose-induced expression of proinflammatory cytolkines in human monocytic THP-1 cells
作者: Wu, C.H.
Wu, C.F.
Huang, H.W.
Jao, Y.C.
Yen, G.C.
關鍵字: Advanced glycation endproducts;CM L;Flavonoids;Glucose;Oxidative;stress;glycation end-products;factor-kappa-b;oxidative stress;cyclooxygenase-2 expression;diabetic complications;molecular-mechanisms;mononuclear-cells;cellular receptor;bcl-2;expression;rage
Project: Molecular Nutrition & Food Research
期刊/報告no:: Molecular Nutrition & Food Research, Volume 53, Issue 8, Page(s) 984-995.
Activation of circulating monocytes by hyperglycemia is bound to play a role in inflammatory and atherosclerosis. In this study, we examined whether flavonoids (catechin, EGCG, luteolin, quercetin, rutin) - phytochemicals that may possible belong to a new class of advanced glycation end products (AGEs) inhibitors - can attenuate high glucose (15 mmol/L, HG)-induced inflammation in human monocytes. Our results show that all flavonoids significantly inhibited HG-induced expression of proinflammatory genes and proteins, including TNF-alpha, interleukin-1 beta (IL-1 beta), and cyclooxygenase (COX)-2, at a concentration of 20 mu M. Flavonoids also prevented oxidative stress in activated monocytes, as demonstrated by their inhibitory effects on intracellular reactive oxygen species (ROS) and N(epsilon)-(carboxymethyl)lysine formation caused by HG. These inhibitory effects may involve inhibition of nuclear factor-kappa B activation and may be supported by downregulation of the following: i) PKC-dependent NADPH oxidase pathway; ii) phosphorylation of p38 mitogen-activated protein kinase and extracellular signal-regulated protein kinase, and iii) mRNA expression of receptor of AGEs. In addition, we found for the first time that lower levels of Bcl-2 protein under HG conditions could be countered by the action of flavonoids. Our data suggest that, along with their antioxidant activities, flavonoids possess anti-inflammatory properties and might therefore have additional protective effects against glycotoxin-related inflammation.
ISSN: 1613-4125
DOI: 10.1002/mnfr.200800495
Appears in Collections:食品暨應用生物科技學系

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