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|標題:||Development of Real-Time Pcr Detection Techniques for Lactic Acid Bacterial Strains to Be Used as Feed Probiotics
A major concern in the antibiotic residues in meat and poultry products has enforced the development of alternatives to feed antibiotics, and a lot of probiotic products containing lactic acid bacteria have been commercialized and used in feed industry. However, the standard and reliable detection methods for lactic acid bacteria in complicated microbial flora are still not available, and there is an urgent need to develop such methods for the accurate identification of commercial lactic acid bacteria intended used for probiotics in the imported or domestic feed additives.Molecular techniques for the detection of bacterial species especially the polymerase chain reaction (PCR) have been rapid developed in recent years. Among them, the application of real-time polymerase chain reaction (real-time PCR) is increasing tremendously and has been proven to be a promising tool in rapid quantitative detection of bacteria with high accuracy, sensitivity and efficiency. In this study, the Real-time PCR quantitative detection technique will be developed using the PCR primers specific to the DNA sequence of lactic acid bacteria commercially used as feed probiotic additives. It is expected that the developing technique will provide rapid and accurate identification for the detection and effective management of lactic acid bacteria in feed additives.
|Appears in Collections:||生物科技學研究所|
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