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標題: 人造油體之生物技術應用開發
Biotechnological Applications Based on Artificial Oil Bodies
作者: 曾志正
關鍵字: 食品科技;應用研究;人造油體;油體特有蛋白質;抗體生產;包埋技術;粉末與錠劑化技術
Plant seeds store neutral lipids, mainly triacylglycerols in intracellular particles termed oil bodies as energy sources for a forthcoming active period of metabolism. Seed oil bodies contain a triacylglycerol matrix surrounded by a layer of phospholipids and unique proteins. In the past years, we successfully established the technique for the constitution of artificial oil bodies and intended to develop several biotechnological applications. Seeing the limitation of a single laboratory, we intend to cooperate with experts in different research areas to expand the applications of artificial oil bodies. We proposed to establish three biotechnological platforms in the following three years. (1)To engineer and produce recombinant oleosin or caleosin for the encapsulation of artificial oil bodies: For a better stability in long-term storage, techniques will be developed for solidification of artificial oil bodies in the forms of powder or tablet. (2) To engineer and produce recombinant caleosin for the encapsulation of artificial oil bodies containing functional antioxidant ingredient, curcumin or an anti-cancer drug, camptothecin. The compounds will be tested for incorporation into triacylglycerol homogenously and then constituted into artificial oil bodies. Bioavailability will be evaluated in rats. (3) To engineer and produce recombinant oleosin or caleosin for the encapsulation of artificial oil bodies to generate antibodies against small molecules, such as catechin ECGC by presenting it on their surface. Different conditions will be tested for the covalent linkage between oleosin/caleosin and the compound. The new engineered oil-body protein will serve as a platform for the production of antibodies against small compounds.

其他識別: NSC100-2313-B005-012-MY3
Appears in Collections:生物科技學研究所

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