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標題: Optimization of lipase-catalyzed biodiesel by isopropanolysis in a continuous packed-bed reactor using response surface methodology
作者: Chang, C.
誠, 張
Chen, J.H.
Chang, C.M.J.
Wu, T.T.
Shieh, C.J.
關鍵字: candida-antarctica lipase;cotton seed oil;transesterification;alcoholysis;solvent
Project: New Biotechnology
期刊/報告no:: New Biotechnology, Volume 26, Issue 3-4, Page(s) 187-192.
Isopropanolysis reactions were performed using triglycerides with immobilized lipase in a solvent-free environment. This study modeled the degree of isopropanolysis of soybean oil in a continuous packed-bed reactor when Novozym (R) 435 was used as the biocatalyst. Response surface methodology (RSM) and three-level-three-factor Box-Behnken design were employed to evaluate the effects of synthesis parameters, reaction temperature (degrees C), flow rate (mL/min) and substrate molar ratio of isopropanol to soybean oil, on the percentage molar conversion of biodiesel by transesterification. The results show that flow rate and temperature have a significant effect on the percentage of molar conversion. On the basis of ridge max analysis, the optimum conditions for synthesis were as follows: flow rate 0.1 mL/min, temperature 51.5 degrees C and substrate molar ratio 1:4.14. The predicted value was 76.62 +/- 1.52% and actual experimental value was 75.62 +/- 0.81% molar conversion. Moreover, continuous enzymatic process for seven days did not show any appreciable decrease in the percent of molar conversion (75%). This work demonstrates the applicability of lipase catalysis to prepare isopropyl esters by transesterification in solvent-free system with a continuous packed-bed reactor for industrial production.
ISSN: 1871-6784
DOI: 10.1016/j.nbt.2009.07.010
Appears in Collections:生物科技發展中心

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