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標題: Effects of using various bioreactors on chitinolytic enzymes production by Paenibacillus taichungensis
作者: Chen, H.B.
Kao, P.M.
Huang, H.C.
Shieh, C.J.
Chen, C.I.
Liu, Y.C.
關鍵字: Oxygen transfer rate;Shear rate;Chitinolytic enzyme production;Paenibacillus taichungensis;Net-draft tube air-lift reactor (ALNR);net-draft-tube;mass-transfer;airlift reactor;sp che-n1;trichoderma-harzianum;chitinase production;bubble-columns;optimization;purification;endochitinase
Project: Biochemical Engineering Journal
期刊/報告no:: Biochemical Engineering Journal, Volume 49, Issue 3, Page(s) 337-342.
In this study, various types of reactors were employed to investigate chitinolytic enzymes production via Paenibacillus taichungensis. It was found that the net-draft tube air-lift reactor (ALNR) gave the best performance among the reactors. Different aeration conditions of ALNR were carried out to evaluate cell growth and chitinolytic enzymes production. It was found that a 2 vvm aeration produces an optimal chitinolytic enzymes activity level of 21.1 mU/mL, which was 2.6 times greater than that obtained in a stirred-tank reactor. To explore the result, the shear rates and oxygen transfer rates of both reactors were evaluated and compared. Meanwhile, the morphologies of the strain for chitinolytic enzymes production were also investigated. (C) 2010 Elsevier B.V. All rights reserved.
ISSN: 1369-703X
DOI: 10.1016/j.bej.2010.01.008
Appears in Collections:生物科技發展中心

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