Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/63345
標題: Studies of optimum conditions for covalent immobilization of Candida rugosa lipase on poly(gamma-glutamic acid) by RSM
作者: Chang, S.W.
謝淳仁
Shaw, J.F.
Yang, K.H.
Chang, S.F.
Shieh, C.J.
關鍵字: Candida rugosa lipase;covalent binding;immobilization;optimization;poly(gamma-glutamic acid);poly(l-glutamic acid);nanoparticles;chitosan;esterification;phenylalanine;membranes;carrier
Project: Bioresource Technology
期刊/報告no:: Bioresource Technology, Volume 99, Issue 8, Page(s) 2800-2805.
摘要: 
Poly(gamma-glutamic acid) (gamma-PGA) is a material of polymer. Immobilization of Candida rugosa lipase (Lipase AY-30) by covalent binding on,gamma-PGA led to a markedly improved performance of the enzyme. Response surface methodology (RSM) and 3-level-3-factor fractional factorial design were employed to evaluate the effects of immobilization parameters, such as immobilization time (2-6 h), immobilization temperature (0-26 degrees C), and enzyme/support ratio (0.1-0.5, w/w). Based on the analysis of ridge max, the optimum immobilization conditions were as follows: immobilization time 2.3 h, immobilization temperature 13.3 degrees C, and enzyme/support ratio 0.41 (w/w); the highest lipase activity obtained was 1196 U/mg-protein. (c) 2007 Elsevier Ltd. All rights reserved.
URI: http://hdl.handle.net/11455/63345
ISSN: 0960-8524
DOI: 10.1016/j.biortech.2007.06.020
Appears in Collections:生物科技發展中心

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