Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/63352
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dc.contributor.authorKuo, Chia-Hungen_US
dc.contributor.authorChiang, Shu-Huaen_US
dc.contributor.authorJu, Hen-Yien_US
dc.contributor.authorChen, Yu-Minen_US
dc.contributor.authorLiao, Ming-Yuanen_US
dc.contributor.authorLiu, Yung-Chuanen_US
dc.contributor.authorShieh, Chwen-Jenen_US
dc.date2012-08zh_TW
dc.date.accessioned2014-06-09T07:05:07Z-
dc.date.available2014-06-09T07:05:07Z-
dc.identifier.urihttp://hdl.handle.net/11455/63352-
dc.description.abstractBACKGROUND: 2-Phenylethyl acetate (2-PEAc) is a highly valued natural volatile ester with a rose-like odour that is widely used to add scent or flavour to cosmetics, soaps, foods and drinks. In this study, 2-PEAc was synthesised enzymatically by transesterification of vinyl acetate with 2-phenethyl alcohol catalysed by immobilised lipase (Novozym 435) from Candida antarctica. RESULTS: Response surface methodology and a three-level/three-factor Box–Behnken design were used to evaluate the effects of time, temperature and enzyme amount on the molar conversion % of 2-PEAc. The results showed that temperature was the most important variable. Based on the ridge max analysis results, optimum enzymatic synthesis conditions were predicted as a reaction time of 79 min, a temperature of 57.8 ◦C and an enzyme amount of 122.5 mg. The predicted and experimental yields were 86.4 and 85.4% respectively. CONCLUSION: Three immobilised lipases were screened and 15 reaction conditions were tested in order to find the combination for maximum yield. The optimisation of 2-PEAc synthesis catalysed by Novozym 435 was successfully developed. The kinetic study of this transesterification reaction showed that it followed an ordered ping-pong bi-bimechanism without any inhibition by reactants.en_US
dc.language.isoen_USzh_TW
dc.relationJ Sci Food Agric, Volume 92, Page(s) 2141–2147.en_US
dc.relation.urihttp://dx.doi.org/10.1002/jsfa.5599en_US
dc.subjectlipaseen_US
dc.subject2-phenylethyl acetateen_US
dc.subjectkineticsen_US
dc.subjectacetylationen_US
dc.subjecttransesterificationen_US
dc.titleEnzymatic synthesis of rose aromatic ester (2-phenylethyl acetate) by lipaseen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1002/jsfa.5599zh_TW
dc.contributor.catalogerWei Chun Wangen_US
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
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