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|dc.description.abstract||BACKGROUND: 2-Phenylethyl acetate (2-PEAc) is a highly valued natural volatile ester with a rose-like odour that is widely used to add scent or flavour to cosmetics, soaps, foods and drinks. In this study, 2-PEAc was synthesised enzymatically by transesterification of vinyl acetate with 2-phenethyl alcohol catalysed by immobilised lipase (Novozym 435) from Candida antarctica. RESULTS: Response surface methodology and a three-level/three-factor Box–Behnken design were used to evaluate the effects of time, temperature and enzyme amount on the molar conversion % of 2-PEAc. The results showed that temperature was the most important variable. Based on the ridge max analysis results, optimum enzymatic synthesis conditions were predicted as a reaction time of 79 min, a temperature of 57.8 ◦C and an enzyme amount of 122.5 mg. The predicted and experimental yields were 86.4 and 85.4% respectively. CONCLUSION: Three immobilised lipases were screened and 15 reaction conditions were tested in order to find the combination for maximum yield. The optimisation of 2-PEAc synthesis catalysed by Novozym 435 was successfully developed. The kinetic study of this transesterification reaction showed that it followed an ordered ping-pong bi-bimechanism without any inhibition by reactants.||en_US|
|dc.relation||J Sci Food Agric, Volume 92, Page(s) 2141–2147.||en_US|
|dc.title||Enzymatic synthesis of rose aromatic ester (2-phenylethyl acetate) by lipase||en_US|
|dc.contributor.cataloger||Wei Chun Wang||en_US|
|Appears in Collections:||生物科技發展中心|
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