Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/63355
標題: Optimization of Lipase-Catalyzed Synthesis of Cetyl Octanoate in Supercritical Carbon Dioxide
作者: Kuo, Chia-Hung
Ju, Hen-Yi
Chu, Shuan-Wei
Chen, Jiann-Hwa
Chieh-Ming, J.Chang
Liu, Yung-Chuan
Shieh, Chwen-Jen
關鍵字: Cetyl octanoate;Esterification;Lipase-catalyzed;Response surface methodology (RSM);Supercritical carbon dioxide (SC-CO2)
Project: J Am Oil Chem Soc, Volume 89, Page(s) 103–110.
摘要: 
Cetyl octanoate, a wax ester of 24 carbons, is
widely used in the cosmetic industry as a base oil. The
current work focuses on lipase-catalyzed synthesis of
cetyl octanoate in supercritical carbon dioxide (SC-CO2)
by esterification of cetyl alcohol and octanoic acid. Three
immobilized lipases were screened, and 15 reaction conditions
were tested in order to find the combination for
maximal yield. The results showed that Novozym 435
was the best catalyst for the synthesis, and a reaction
time of 20 min was adequate for a maximal yield.
Response surface methodology (RSM) with a 3-factor-
3-level Box-Behnken design was employed to evaluate
the effects of synthesis parameters, including reaction
temperature (35–75 C), pressure (8.27–12.41 MPa), and
enzyme amount (5–15% wt of cetyl alcohol). A model for
the synthesis was developed and the optimum conditions
could be predicted to be reaction pressure of 10.22 MPa,
reaction temperature of 63.70 C, and enzyme amount of
11.20%. An experiment was performed under this optimum
condition and a yield of 99.5% was obtained. This
experimental yield correlated well with the predicted
value of yield (97.6%). We concluded that, in a SC-CO2
system, nearly 100% molar conversion of cetyl octanoate
could be obtained by immobilized Novozym 435 in a
short reaction time (20 min) under the predicted optimal
conditions.
URI: http://hdl.handle.net/11455/63355
DOI: 10.1007/s11746-011-1895-8
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