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標題: High Yield of Wax Ester Synthesized from Cetyl Alcohol and Octanoic Acid by Lipozyme RMIM and Novozym 435
作者: Kuo, Chia-Hung
Chen, Hsin-Hung
Chen, Jiann-Hwa
Liu, Yung-Chuan
Shieh, Chwen-Jen
關鍵字: optimization;wax esters;lipase;esterification;Rhizomucor miehei;Candida antarctica
Project: Int. J. Mol. Sci., Volume 13, Page(s) 11694-11704.
Wax esters are long-chain esters that have been widely applied in premium
lubricants, parting agents, antifoaming agents and cosmetics. In this study, the biocatalytic
preparation of a specific wax ester, cetyl octanoate, is performed in n-hexane using two
commercial immobilized lipases, i.e., Lipozyme® RMIM (Rhizomucor miehei) and
Novozym® 435 (Candida antarctica). Response surface methodology (RSM) and
5-level-4-factor central composite rotatable design (CCRD) are employed to evaluate the
effects of reaction time (1–5 h), reaction temperature (45–65 °C), substrate molar ratio
(1–3:1), and enzyme amount (10%–50%) on the yield of cetyl octanoate. Using RSM to
optimize the reaction, the maximum yields reached 94% and 98% using Lipozyme® RMIM
and Novozym® 435, respectively. The optimum conditions for synthesis of cetyl octanoate
by both lipases are established and compared. Novozym® 435 proves to be a more efficient
biocatalyst than Lipozyme® RMIM.
ISSN: 1422-0067
DOI: 10.3390/ijms130911694
Appears in Collections:生物科技發展中心

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