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Population genetic variation of Eriobotrya in Taiwan
The purpose of the study was to determine the genetic differences among the different populations of Eriobotrya deflaxa over 10 populations using Random amplified polymorphism DNA(RAPD)technique. The Kobayashi method was better at purifying and concentrating the DNA than the standard CTAB method. A total of 144 individuals were collected from 10 populations and 4 regions in Taiwan. Thirteen primers having 186 bands were found. Of the bands 106(56.9﹪)were monomorphic and 80(43.1﹪) were polymorphic. The phenology of the flowering period among different Eriobotrya deflexa populations overlapped , No obvious reproductive isolation based on geography could be found.
The result based on the principle of coordinate analysis(PCA) and UPGMA clustering showed that the populations at Eranbic, Chi-niu ling, and Kenting park were all Eribotrya deflexa form. koshunensis.. The populations at Yang-Ming Mountain, Da-Yu ling, Li-Sang and Tian-xiang were found to be Eriobotrya deflexa f. deflexa. Northern Daiwu Mountain and Da-Hang Mountain belong to Eriobotrya deflexa f. buisanensis, but it's genetic distance is close to Eriobotrya japonica and far from the other populations. It is better treated as a species or subspecies. AMOVA analysis revealed that the variance component among groups to be 16.56﹪ the variance component among populations occupied 36.16﹪, and the variance within populations occupied 47.29﹪. The averages of genetic diversity and DNA polymorphic fingerprinting in Eriobotrya deflexa form koshunensis are higher than other groups, indicating much higher differentiation..The genetic diversity is 0.338 and gene flow is 0.9791.
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