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標題: 柳杉葉部精油對小鼠中樞神經系統影響及口服急毒性試驗評估
Effect of the Essential Oil from Cryptomeria japonica Leaves on the Central Nervous System and Acute Oral Toxicity Evaluation in Mice
作者: 鄭崴文
Cheng, Wei-Wen
關鍵字: 柳杉;Cryptomeria japonica;精油;神經藥理學活性;芬多精;口服急毒性;essential oil;neuropharmacological activities;phytoncide;acute oral toxicity test
出版社: 森林學系所
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森林浴(green shower;shinrin-yoku)對人類的健康具有許多之益處,本研究乃針對柳杉林內所釋放出來的森林芬多精,利用微固相萃取法以及氣相層析儀進行組成分分析。分析結果顯示,柳杉林內所釋放出之芬多精之主要成分為α-pinene (19.35%)、β-myrcene (16.98%)、d-limonene (15.21%)以及γ-muurolene (7.42%);精油之主要成分為elemol (18.22%)、16-kaurene (11.63%)、3-carene (9.66%)、sabinene (9.37%)、4-terpineol (9.06%)、α-eudesmol (5.70%)、α-pinene (5.62%)以及d-limonene (5.26%)。此外,本研究續利用數種動物行為模型來評估柳杉葉部精油及其重要之單萜類,d-limonene,對小鼠中樞神經系統的作用與影響。結果發現柳杉葉部精油具有明顯的延長戊巴比妥誘導小鼠之睡眠持續時間;在高腳十字迷宮試驗與冰醋酸扭體試驗中也顯示,柳杉葉部精油與d-limonene皆具有抗焦慮及鎮痛之功用;並且可平緩咖啡因誘導的興奮情形,以及具有保護小鼠受到戊四唑驚厥之傷害。
為探討柳杉葉部精油使用之安全性,本研究以ICR品系小鼠進行口服急毒性試驗。試驗結果顯示,柳杉葉部精油對小鼠口服急毒性之半致死劑量(LD50)為9.83 g/kg B.W.,且經精油處理小鼠之腦、心、肝、腎、脾及胸腺等重要臟器,均無因試驗物質引起肉眼及組織病理變化;血液學檢測中之紅血球數、血紅素、血球容積比、平均血紅素濃度及血小板等相較於對照組均無影響;血清生化檢測中之麩氨酸氨基轉氨酶、麩氨酸丙酸轉氨酶與肌氨酸等相較於對照組亦沒有影響(p>0.05)。

Forest-air bathing and walking (green shower, shinrin-yoku) is beneficial on human health. In this study the phytoncide released from Cryptomeria japonica plantation forestry was collected by using solid-phase micro extraction (SPME) and followed by GC/MS analysis, the composition of phytoncide was characterized. The main compositions of phytoncide were α-pinene (19.35%), β-myrcene (16.98%), d-limonene (15.21%) and γ-muurolene (7.42%). On the other hand, the essential oil of C. japonica was obtained by water distillation and anglicized by GC/MS, its main composition were elemol (18.22%)、16-kaurene (11.63%)、3-carene (9.66%)、sabinene (9.37%)、4-terpineol (9.06%)、α-eudesmol (5.70%)、α-pinene (5.62%), and d-limonene (5.26%). Furthermore, the neuropharmacological activity of the essential oils from leaves of C. japonica (ECJ) was evaluated by several animal behavior tests. ECJ could prolong the sleeping phase of ICR mice in pentobarbital-induced sleeping time model. Besides, both EJC and one of its monoterpene, d-limonene possessed the potent anxiolytic and analgestic.activities based on the results obtained from elevated plus-maze and writhing tests. The formulation also antagonized the behavioral effects of CNS-stimulant drug caffeine, and protected mice from PTZ induced convulsions.
To evaluate the safety of EJC, the acute oral toxicity test in ICR mice was conduct in this study. The LD50 of EJC was estimated 9.83 g/kg body weight for mice. No significant lesions of brain, heart, liver, kidney, spleen and thymus were found at gross and histopathological observation in the treated mice. Hematological parameters included of RBC, HGB, HCT, MCH, and PLT values were as normal as control. Besides, serum biochemistry changes in liver and renal function included of ALT, AST and creatinine values had no significant difference (p > 0.05) with control.
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