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Caprine Arthritis Encephalitis of Surveillance,Prevention and Control in Taiwan
Song, Ming Hwa
|關鍵字:||Caprine Arthritis Encephalitis;山羊關節炎腦炎;Transmission;Serosurvelliance;Prevention;血清監控;傳播;預防||出版社:||獸醫微生物學研究所||摘要:||
中 文 摘 要
山羊關節炎腦炎(Caprine arthritis encephalitis ;CAE)為慢性消
隻形 成關節腫大病症，而其中58.6%的關節腫大羊隻在2歲時被畜 主
在傳播途徑方面，集乳(pooled milk)方式為本病最主 要傳播方式。水
敏感性為99%，相對 特異性為91.3%，相對一致性為95.4%，Kappa 值
Caprine arthritis encephalitis (CAE) is one kind of chronic
wasting disease which causes great financial losses in dairy
goat industry. The disease has been spread worldwidely
including Taiwan. In this research we worked on the
serological surveillance, blockade of
transmission route, and establishment of ELISA test and
tried to construct a model for CAE free goat farm.
From 1988 to 1996, we collected 1170 serum samples from
27 goat farms for CAE serological study using ELISA method.
The results indicated that all of the farms were contaminated
with CAE virus and the positive samples were 691 (59.1%).
Through the observation of swollen joints of goats in
the field compared to CAE antibody, there was a remarkable
correlation between both factors (p<0.01). We further
questionnaired 23 dairy goat farms for the economic loss
caused by swollen joints. Those results revealed that
there was 12.3% of goats showing swollen joints within
one year, and 58.6% of those goats at the age of 2 culled
by raiser. Matching study of the milking performance and CAE
virus infection in one dairy goat farm showed CAE virus infected
goats decreasing 0.34 liter milk production per goat per day
compared to uninfected goats, which was equal to 13.7%
economic loss of net income.
Pooled milking was the major transmission route in CAE
transmission of goat kids, but the horizontal route was also
important in young and adult goats when they were raised in poor
environmental condition. The CAE seronegative goats living
with CAE seropositive goats for one year showed 33.3%
seroconversion rate. No obvious evidence proved the
transmission of the disease was through mating and
transplacental route. In vitro study indicated that the
infectivity of the virus in milk was lost after heated the milk
to 56℃for an hour.
In this study we aslo established ELISA method for CAE
antibody detection. After box titration, the proper virus
protein concentration was 4mg/ml. Compared ELISA method with
Western blotting, there was a relative high correlation between
them. From the comparison of 194 serum samples, the relative
sensitivity was 99%, the relative specificity was 91.3% and the
correlation of both methods was 95.4%.
According to above results, we followed the serological
surveillance on dairy goat farms which heated pooled colostrum
to feed kids and separate kids from their dams after born. The
average seroconversion rates of goats, which were separated
from their dams in different house and pens at the age of to 11
months, were 15.2% and 24.5%, respectively. In contrast goat
kids fed with unheated colostrum and only separated from their
dams in different pens showed 81.3% positive for CAEV
infection at the age of 8 months.
The above results indicated that Taiwan has become a high
prevalent area of CAE virus infection which potentially causes a
large amount of financial losses. It is the main transmission
route of the disease to feed untreated pooled milk for kids, and
living together second. ELISA is a sensitive and rapid monitor
method for CAE virus infection. Heated colostrum and
separated CAE seronegative goats from positive goats can
efficiently reduce the infection.
|Appears in Collections:||微生物暨公共衛生學研究所|
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