Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66330
標題: 台灣中部地區羊隻屠體表面微生物品質調查
Microbiological quality survey of goat carcasses in Central Taiwan
作者: 林基斌
Lin, Chi-Pin
關鍵字: carcasses;屠體;Microbiological quality;微生物品質
出版社: 獸醫公共衛生學研究所
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摘要: 
本研究旨在調查台灣中部地區羊隻屠體微生物品質,於2004年6月至2005年6月間,依USDA/ FSIS規範方法採樣,採樣期間視該場當日屠宰量以便利抽樣法選定6~20頭屠體採樣,以海棉擦拭法蒐集屠體表面檢體,期間共採樣25次蒐集404個屠體表面檢體,分別為剖半後(n=314)、刮毛後(n=50)及燙毛後(n=40)。檢體皆以平板計數培養基 (Plate count agar)及酵素色質性培養基(Chromocult coliform agar)檢測其微生物品質,其結果總生菌數、腸內菌群及大腸桿菌數分別為4.01、2.46及0.86 log CFU/cm2,檢出率為97.1%、87.3%及41.4%。檢體菌數並採用SPSS軟體進行分析,以Kruskal-Wallis法分析不同採樣點微生物品質,結果在刮毛採樣點菌數最低具顯著差異 (p<0.05);另比較不同採樣點檢出率、菌數平均值皆呈V字型分佈,亦即刮毛後屠體微生物品質最佳。另以Mann-Whitney U檢定不同季節間微生物品質,結果在冷、暖季節間顯著差異(p<0.05)。本調查在總生菌數、腸內菌群、大腸桿菌數結果皆以燙毛後採樣點最高。顯示羊屠體刮毛前的沖洗較燙毛更有效降低菌數。再者屠體微生物品質在不同季節的差異,發現腸內菌群及大腸桿菌檢出率及Mann-Whitney U法檢定結果,皆夏季高於其他季節。另所有檢體皆檢測沙門氏桿菌,惟檢出率為零。另以複合式聚合酶鏈鎖反應檢測病原性大腸桿菌檢出率雖僅為2.22%,顯示羊屠體仍具病原性大腸桿菌污染風險。綜以本調查認為該屠宰場燙毛程序為其重要管制點,應確實監控燙毛程序的危害(如水溫與清潔),以降低屠體遭病原菌污染的風險,俾樹立國產畜產品安全衛生的形象。

The aim of this research is to investigate the microbiological quality of goat carcasses in central Taiwan. Samples were collected in accordance with USDA/FSIS standardized method from June 2004 to June 2005. Depending on the quantities of the slaughtered goat of the sampling day, six to twenty carcasses were selected by convenience sampling method. The carcass was wiped with sponge to collect the surface microbes. There are 25 sampling with 404 Swab samples collections. The sampling numbers from split, scrape and polish types were 314, 50, and 40, respectively. Collected samples were cultured on plate count agar, and coliform agar to examine the microbiological quality. The log mean numbers of total count, coliform, and Escherichia coli were 4.01, 2.46, 0.86 log CFU/ cm2, respectively. Whereas the prevalence were 97.1%, 87.3%,and 41.4%, respectively. The differences of sampling count were analyzed using Kruskal-Wallis test, which was performed with statistical software SPSS to compare the microbial quality from different sampling types. Result shown that the count of scrape type is the lowest and the difference is most significant (p<0.05); Besides, prevalence and the mean of total count, coliform, and Escherichia coli from different sampling types were V-shaped distribution, which means the microbiological quality of scrape type is the best. Mann-Whitney U was used to examine the microbiological quality in different seasons. Result showed that there was significant difference between cold and warm season (p<0.05). The investigation result of polish type in total count, coliform and Escherichia coli were highest. It demonstrated that the rinsing before scrape of goat carcass is much effectively to reduce the microbial count than polish of goat carcass. Moreover, examination of microbial quality of carcass, coliform and Escherichia coli prevalence of carcass, and Mann-Whitney U method among different seasons, it had demonstrated summer's count was highest than those of the other seasons. All samples were examined for the count of Salmonella, yet the prevalence was zero. Moreover, pathogenic Escherichia coli were examined by Multiplex PCR. Although the prevalence is only 2.22%, the goat carcass still bears the risk of contamination by pathogenic Escherichia coli. To summarize, it is believed in this investigation that the polish procedures in slaughterhouse shall be the critical control point. The hazard of the polish procedures, such as water temperature and sanitary, shall be monitored in order to decrease the risk of pollution of pathogens and to establish the value of “National animal products is the representation of safety and hygiene”.
URI: http://hdl.handle.net/11455/66330
其他識別: U0005-2407200617015200
Appears in Collections:微生物暨公共衛生學研究所

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