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標題: 雞傳染性鼻炎重組次單位疫苗之研發
Development of recombinant subunit vaccines for infectious coryza
作者: 潘彥江
Pan, Yen-Chiang
關鍵字: Avibacterium paragallinarum;雞傳染性鼻炎菌;infectious coryza;RTX toxins;HA protein;vaccine;傳染性可利查;RTX 毒素;血球凝集蛋白;疫苗
出版社: 微生物暨公共衛生學研究所
引用: 林茂勇,宋華聰。2006。禽病診治(第二版)。藝軒圖書出版社。台北市。中華民國。2,177-180。 陳秉誼。2009。雞傳染性鼻炎菌莢膜生合成基因之分析與重組次單位疫苗之研究。國立中興大學獸醫微生物學研究所。 Abascal, E.N., Guerra, A.C., Vazquez, A.S., Tenorio, V.R., Cruz, C.V., Zenteno, E., Contreras, G.P., Pacheco, S.V., 2009, Identification of iron-acquisition proteins of Avibacterium paragallinarum. Avian Pathol 38, 209-213. Barnard, T.G., Van Heerden, E., Bragg, R.R., Albertyn, J., 2008, Haemophilus paragallinarum haemagglutinin: role in adhesion, serotyping and pathogenicity. Onderstepoort J Vet Res 75, 11-16. Bisgaard, M., Christensen, J.P., Bojesen, A.M., Christensen, H., 2007, Avibacterium endocarditidis sp. nov., isolated from valvular endocarditis in chickens. Int J Syst Evol Microbiol 57, 1729-1734. Blackall, L.L., 1995, Vaccines against infectious coryza. Blackall, P.J., 1991, An Evaluation of the Cross-Protection Afforded by Inactivated Infectious Coryza Vaccines. Aust Vet J 68, 266-267. Blackall, P.J., 1999, Infectious coryza: Overview of the disease and new diagnostic options. Clin Microbiol Rev 12, 627-+. Blackall, P.J., Christensen, H., Beckenham, T., Blackall, L.L., Bisgaard, M., 2005, Reclassification of Pasteurella gallinarum, [Haemophilus] paragallinarum, Pasteurella avium and Pasteurella volantium as Avibacterium gallinarum gen. nov., comb. nov., Avibacterium paragallinarum comb. nov., Avibacterium avium comb. nov. and Avibacterium volantium comb. nov. Int J Syst Evol Microbiol 55, 353-362. Blackall, P.J., Eaves, L.E., Aus, G., 1990a, Serotyping of Haemophilus paragallinarum by the Page scheme: comparison of the use of agglutination and hemagglutination-inhibition tests. Avian Dis 34, 643-645. Blackall, P.J., Eaves, L.E., Rogers, D.G., 1989, Biotyping of Haemophilus paragallinarum isolates using hemagglutinin serotyping, carbohydrate fermentation patterns, and antimicrobial drug resistance patterns. Avian Dis 33, 491-496. Blackall, P.J., Eaves, L.E., Rogers, D.G., 1990b, Proposal of a new serovar and altered nomenclature for Haemophilus paragallinarum in the Kume hemagglutinin scheme. J Clin Microbiol 28, 1185-1187. Blackall, P.J., Morrow, C.J., McInnes, A., Eaves, L.E., Rogers, D.G., 1990c, Epidemiologic studies on infectious coryza outbreaks in northern New South Wales, Australia, using serotyping, biotyping, and chromosomal DNA restriction endonuclease analysis. Avian Dis 34, 267-276. Blackall, P.J., Reid, G.G., 1987, Further efficacy studies on inactivated, aluminum-hydroxide-adsorbed vaccines against infectious coryza. Avian Dis 31, 527-532. Blackall, P.J., Rogers, D.G., Yamamoto, R., 1990d, Outer-membrane proteins of Haemophilus paragallinarum. Avian Dis 34, 871-877. Blackall, P.J., Yamaguchi, T., Iritani, Y., Rogers, D.G., 1990e, Evaluation of two monoclonal antibodies for serotyping Haemophilus paragallinarum. Avian Dis 34, 861-864. Boshoff, C.H., Coetzee, L., Visser, L., Verschoor, J.A., 1992, Spontaneous hybridoma formation induced by immunization with Haemophilus paragallinarum: evidence for a lipopolysaccharide fusion inducer. Hybridoma 11, 257-266. Boulnois, G.J., Roberts, I.S., 1990, Genetics of capsular polysaccharide production in bacteria. Curr Top Microbiol Immunol 150, 1-18. Bragg, R.R., 2002, Isolation of serovar C-3 Haemophilus paragallinarum from Zimbabwe: A further indication of the need for the production of vaccines against infectious coryza containing local isolates of H. paragallinarum. Onderstepoort J Vet Res 69, 129-132. Bragg, R.R., 2004, Limitation of the spread and impact of infectious coryza through the use of a continuous disinfection programme. Onderstepoort J Vet Res 71, 1-8. Bragg, R.R., 2005, Effects of differences in virulence of different serovars of Haemophilus paragallinarum on perceived vaccine efficacy. Onderstepoort J Vet Res 72, 1-6. Bragg, R.R., Greyling, J.M., Verschoor, J.A., 1997, Isolation and identification of NAD-independent bacteria from chickens with symptoms of infectious coryza. Avian Pathol 26, 595-606. Byarugaba, D.K., Minga, U.M., Gwakisa, P.S., Katunguka-Rwakishaya, E., Bisgaard, M., Olsen, J.E., 2007a, Investigations of the occurrence of Avibacterium paragallinarum infections in Uganda. Avian Dis 51, 534-539. Byarugaba, D.K., Minga, U.M., Gwakisa, P.S., Katunguka-Rwakishaya, E., Bisgaard, M., Olsen, J.E., 2007b, Virulence characterization of Avibacterium paragallinarum isolates from Uganda. Avian Pathol 36, 35-42. Cabrera, A., Morales-Erasto, V., Salgado-Miranda, C., Blackall, P.J., Soriano-Vargas, E., 2011, Hemagglutinin serotyping of Avibacterium paragallinarum isolates from Ecuador. Trop Anim Health Prod 43, 549-551. Calderon, E.N., Thomas, K., Morales-Erasto, V., Salgado-Miranda, C., Soriano-Vargas, E., 2010, Identification of Avibacterium paragallinarum serovar B-1 from severe infectious coryza outbreaks in Panama. Avian Dis 54, 1095-1097. Chancellor, M.B., 2001, RTX exotoxins. Urology 57, 106-107. Chen, X., Zhang, P., Blackall, P.J., Feng, W., 1993, Characterization of Haemophilus paragallinarum isolates from China. Avian Dis 37, 574-576. Christensen, H., Blackall, P.J., Bisgaard, M., 2009, Phylogenetic relationships of unclassified, satellitic Pasteurellaceae obtained from different species of birds as demonstrated by 16S rRNA gene sequence comparison. Res Microbiol 160, 315-321. Chukiatsiri, K., Sasipreeyajan, J., Neramitmansuk, W., Chansiripornchai, N., 2009, Efficacy of autogenous killed vaccine of Avibacterium paragallinarum. Avian Dis 53, 382-386. Conde, M.D., Huberman, Y.D., Espinoza, A.M., Delgado, R.I., Terzolo, H.R., 2011, Vaccination of one-day-old broiler chicks against infectious coryza. Avian Dis 55, 119-122. Corney, B.G., Diallo, I.S., Wright, L., Hewitson, G., De Jong, A., Tolosa, X., Burrell, P., Duffy, P., Rodwell, B., Boyle, D.B., Blackall, P.J., 2008, Rapid and sensitive detection of Avibacterium paragallinarum in the presence of other bacteria using a 5'' Taq nuclease assay: a new tool for diagnosing infectious coryza. Avian Pathol 37, 599-604. Czuprynski, C.J., Welch, R.A., 1995, Biological effects of RTX toxins: the possible role of lipopolysaccharide. Trends Microbiol 3, 480-483. Droual, R., Bickford, A.A., Charlton, B.R., Cooper, G.L., Channing, S.E., 1990a, Infectious coryza in meat chickens in the San Joaquin Valley of California. Avian Dis 34, 1009-1016. Droual, R., Bickford, A.A., Charlton, B.R., Kuney, D.R., 1990b, Investigation of problems associated with intramuscular breast injection of oil-adjuvanted killed vaccines in chickens. Avian Dis 34, 473-478. Dungu, B., Brett, B., MacDonald, R., Deville, S., Dupuis, L., Theron, J., Bragg, R.R., 2009, Study on the efficacy and safety of different antigens and oil formulations of infectious coryza vaccines containing an NAD-independent strain of Avibacterium paragallinarum. Onderstepoort J Vet Res 76, 299-309. Eaves, L.E., Rogers, D.G., Blackall, P.J., 1989, Comparison of hemagglutinin and agglutinin schemes for the serological classification of Haemophilus paragallinarum and proposal of a new hemagglutinin serovar. J Clin Microbiol 27, 1510-1513. Fernandez, R.P., Colindres, H.L., Velasquez, Q.E., Soriano, V.E., Blackall, P.J., 2005, Protection conferred by bivalent and trivalent infectious coryza bacterins against prevalent serovars of Avibacterium (Haemophilus) paragallinarum in Mexico. Avian Diseases 49, 585-587. Frey, J., Kuhnert, P., 2002, RTX toxins in Pasteurellaceae. Int J Med Microbiol 292, 149-158. Garcia, A., Romo, F., Ortiz, A.M., Blackall, P.J., 2008, The vaccination-challenge trial: the gold standard test to evaluate the protective efficacy of infectious coryza vaccines. Avian Pathology 37, 183-186. Garcia, A.J., Angulo, E., Blackall, P.J., Ortiz, A.M., 2004, The presence of nicotinamide adenine dinucleotide-independent Haemophilus paragallinarum in Mexico. Avian Dis 48, 425-429. Giurov, B., 1984, [Clinical cases of infectious coryza and the properties of isolated Haemophilus strains]. Vet Med Nauki 21, 22-30. Hallstrom, T., Riesbeck, K., 2010, Haemophilus influenzae and the complement system. Trends Microbiol 18, 258-265. Hinz, K.H., 1973a, [Contribution to the differentiation of haemophilus-strains isolated from chickens. II: Serological examinations using the plate-agglutination test]. Avian Pathol 2, 269-278. Hinz, K.H., 1973b, [Report on the differentiation of Haemophilus isolates from chickens. I. Note: cultural and biochemical studies]. Avian Pathol 2, 211-229. Hinz, K.H., 1980, Heat-stable antigenic determinants of Haemophilus paragallinarum. Zentralbl Veterinarmed B 27, 668-676. Hinz, K.H., Muller, H.E., 1980, [Effect of the neuraminidases of Haemophilus avium and Haemophilus paragallinarum on chicken serum and other substrates]. Avian Pathol 9, 531-537. Hobb, R.I., Tseng, H.J., Downes, J.E., Terry, T.D., Blackall, P.J., Takagi, M., Jennings, M.P., 2002, Molecular analysis of a haemagglutinin of Haemophilus paragallinarum. Microbiology 148, 2171-2179. Hoyle, L., 1934, The Haemophilic Bacteria of the Upper Respiratory Tract, the Appearance of Virulent Forms in Relation to Upper Respiratory Infections. J Hyg (Lond) 34, 195-202. Hsu, Y.M., Shieh, H.K., Chen, W.H., Shiang, J.H., Chang, P.C., 2007a, Immunogenicity and haemagglutination of recombinant Avibacterium paragallinarum HagA. Vet Microbiol 122, 280-289. Hsu, Y.M., Shieh, H.K., Chen, W.H., Sun, T.Y., Shiang, J.H., 2007b, Antimicrobial susceptibility, plasmid profiles and haemocin activities of Avibacterium paragallinarum strains. Vet Microbiol 124, 209-218. Huberman, Y.D., Bueno, D.J., Terzolo, H.R., 2005, Evaluation of the protection conferred by a disinfectant against clinical disease caused by Avibacterium paragallinarum serovars A, B, and C from Argentina. Avian Dis 49, 588-591. Iritani, Y., Iwaki, S., Yamaguchi, T., Sueishi, T., 1981a, Determination of types 1 and 2 hemagglutinins in serotypes of Hemophilus paragallinarum. Avian Dis 25, 479-483. Iritani, Y., Yamaguchi, T., Katagiri, K., Arita, H., 1981b, Hemagglutination inhibition of haemophilus paragallinarum type 1 hemagglutinin by lipopolysaccharide. Am J Vet Res 42, 689-690. Kaur, J., Banga, H.S., Sood, N.K., Gupta, K., Singh, A., 2005, Pathological studies on naturally occurring infectious coryza in chickens. Indian J Anim Sci 75, 209-211. Kaur, J., Sharma, N.S., Gupta, K., Singh, A., 2004, Epidemiological studies on infectious coryza in chickens in northern India. Indian J Anim Sci 74, 462-465. Klein, M., Blazevic, D.J., 1969, Differentiation of haemophilus species using satellite methods. Am J Med Technol 35, 695-701. Kristensen, B.M., Frees, D., Bojesen, A.M., 2010, GtxA from Gallibacterium anatis, a cytolytic RTX-toxin with a novel domain organisation. Vet Res 41, 25. Kuhnert, P., Berthoud, H., Straub, R., Frey, J., 2003, Host cell specific activity of RTX toxins from haemolytic Actinobacillus equuli and Actinobacillus suis. Vet Microbiol 92, 161-167. Kuhnert, P., Heyberger-Meyer, B., Burnens, A.P., Nicolet, J., Frey, J., 1997, Detection of RTX toxin genes in gram-negative bacteria with a set of specific probes. Appl Environ Microbiol 63, 2258-2265. Kume, K., Sawata, A., Nakai, T., 1983a, Serologic and immunologic studies on three types of hemagglutinin of Haemophilus paragallinarum serotype 1 organisms. Nippon Juigaku Zasshi 45, 783-792. Kume, K., Sawata, A., Nakai, T., 1984, Clearance of the challenge organisms from the upper respiratory tract of chickens injected with an inactivated Haemophilus paragallinarum vaccine. Nippon Juigaku Zasshi 46, 843-850. Kume, K., Sawata, A., Nakai, T., Matsumoto, M., 1983b, Serological classification of Haemophilus paragallinarum with a hemagglutinin system. J Clin Microbiol 17, 958-964. Ladant, D., Ullmann, A., 1999, Bordetella pertussis adenylate cyclase: a toxin with multiple talents. Trends in Microbiology 7, 172-176. Lally, E.T., Hill, R.B., Kieba, I.R., Korostoff, J., 1999, The interaction between RTX toxins and target cells. Trends Microbiol 7, 356-361. Leduc, I., Olsen, B., Elkins, C., 2009, Localization of the domains of the Haemophilus ducreyi trimeric autotransporter DsrA involved in serum resistance and binding to the extracellular matrix proteins fibronectin and vitronectin. Infect Immun 77, 657-666. Lin, J.A., Shyu, C.L., Yamaguchi, T., Takagi, M., 1996, Characterization and pathogenicity of Haemophilus paragallinarum serotype C in local chickens of Taiwan. J Vet Med Sci 58, 1007-1009. Linke, D., Riess, T., Autenrieth, I.B., Lupas, A., Kempf, V.A., 2006, Trimeric autotransporter adhesins: variable structure, common function. Trends Microbiol 14, 264-270. Mazar, J., Cotter, P.A., 2006, Topology and maturation of filamentous haemagglutinin suggest a new model for two-partner secretion. Mol Microbiol 62, 641-654. Mena-Rojas, E., Vazquez Cruz, C., Vaca Pacheco, S., Garcia Gonzalez, O., Perez-Marquez, V.M., Perez-Mendez, A., Ibarra-Caballero, J., de la Garza, M., Zenteno, E., Negrete-Abascal, E., 2004, Antigenic secreted proteins from Haemophilus paragallinarum. A 110-kDa putative RTX protein. FEMS Microbiol Lett 232, 83-87. Mendoza-Espinoza, A., Koga, Y., Zavaleta, A.I., 2008, Amplified 16S ribosomal DNA restriction analysis for identification of Avibacterium paragallinarum. Avian Dis 52, 54-58. Miao, D., Zhang, P., Gong, Y., Yamaguchi, T., Iritani, Y., Blackall, P.J., 2000, The development and application of a blocking ELISA kit for the diagnosis of infectious coryza. Avian Pathol 29, 219-225. Moxon, E.R., 2009, Bacterial variation, virulence and vaccines. Microbiology 155, 997-1003. Noro, T., Oishi, E., Kaneshige, T., Yaguchi, K., Amimoto, K., Shimizu, M., 2008, Identification and characterization of haemagglutinin epitopes of Avibacterium paragallinarum serovar C. Vet Microbiol 131, 406-413. Noro, T., Yaguchi, K., Amimoto, K., Oishi, E., 2007, Identification and expression of a gene encoding an epitope that induces hemagglutination inhibition antibody to Avibacterium paragallinarum serovar A. Avian Dis 51, 84-89. Ogunnariwo, J.A., Schryvers, A.B., 1992, Correlation between the ability of Haemophilus paragallinarum to acquire ovotransferrin-bound iron and the expression of ovotransferrin-specific receptors. Avian Dis 36, 655-663. Perez-Marquez, V., Perez-Mendez, A., Ibarra-Caballero, J., Gomez-Lugo, G., Vazquez-Cruz, C., Vaca, S., Negrete-Abascal, E., 2008, Secreted proteins of Avibacterium paragallinarum are lethal for chicken embryo. Ann N Y Acad Sci 1149, 380-383. Piechulla, K., Bisgaard, M., Gerlach, H., Mannheim, W., 1985a, Taxonomy of some recently described avian Pasteurella/Actinobacillus-like organisms as indicated by deoxyribonucleic acid relatedness. Avian Pathol 14, 281-311. Piechulla, K., Hinz, K.H., Mannheim, W., 1985b, Genetic and phenotypic comparison of three new avian Haemophilus-like taxa and of Haemophilus paragallinarum Biberstein and White 1969 with other members of the family Pasteurellaceae Pohl 1981. Avian Dis 29, 601-612. Poernomo, S., Sutarma, Rafiee, M., Blackall, P.J., 2000, Characterisation of isolates of Haemophilus paragallinarum from Indonesia. Aust Vet J 78, 759-762. Ramon Rocha, M.O., Garcia-Gonzalez, O., Perez-Mendez, A., Ibarra-Caballero, J., Perez-Marquez, V.M., Vaca, S., Negrete-Abascal, E., 2006, Membrane vesicles released by Avibacterium paragallinarum contain putative virulence factors. FEMS Microbiol Lett 257, 63-68. Rashid, R.A., Pociecha, J.Z., 1984, Epidemiological study of an outbreak of infectious coryza on a poultry farm in Iraq. Avian Dis 28, 235-237. Rimler, R.B., Shotts, E.B., Jr., Brown, J., Davis, R.B., 1977, The effect of sodium chloride and NADH on the growth of six strains of haemophilus species pathogenic to chickens. J Gen Microbiol 98, 349-354. Rivero-Garcia, P.C., Cruz, C.V., Alonso, P.S., Vaca, S., Negrete-Abascal, E., 2005, Haemophilus paragallinarum secretes metalloproteases. Can J Microbiol 51, 893-896. Roberts, I.S., 1996, The biochemistry and genetics of capsular polysaccharide production in bacteria. Annu Rev Microbiol 50, 285-315. Rogers, R.J., Eaves, L.E., Blackall, P.J., Truman, K.F., 1990, The comparative pathogenicity of four serovars of Actinobacillus (Haemophilus) pleuropneumoniae. Aust Vet J 67, 9-12. Roodt, Y., Bragg, R., Albertyn, J., 2007, The cloning and sequencing of the UDP-galactose 4-epimerase gene (galE) from Avibacterium paragallinarum. DNA Seq 18, 265-268. Rossman, J.S., Lamb, R.A., 2011, Influenza virus assembly and budding. Virology 411, 229-236. Sasaki, H., Kawamoto, E., Tanaka, Y., Sawada, T., Kunita, S., Yagami, K., 2009, Identification and characterization of hemolysin-like proteins similar to RTX toxin in Pasteurella pneumotropica. J Bacteriol 191, 3698-3705. Sawata, A., Kume, K., 1983, Relationships between virulence and morphological or serological properties of variants dissociated from serotype 1 Haemophilus paragallinarum strains. J Clin Microbiol 18, 49-55. Sawata, A., Kume, K., Nakai, T., 1984a, Relationship between anticapsular antibody and protective activity of a capsular antigen of Haemophilus paragallinarum. Nippon Juigaku Zasshi 46, 475-486. Sawata, A., Kume, K., Nakai, T., 1984b, Susceptibility of Haemophilus paragallinarum to bactericidal activity of normal and immune chicken sera. Nippon Juigaku Zasshi 46, 805-813. Sawata, A., Nakai, T., Kume, K., Yoshikawa, H., Yoshikawa, T., 1985, Intranasal inoculation of chickens with encapsulated or nonencapsulated variants of Haemophilus paragallinarum: electron microscopic evaluation of the nasal mucosa. Am J Vet Res 46, 2346-2353. Shivaprasad, H.L., Droual, R., 2002, Pathology of an atypical strain of Pasteurella gallinarum infection in chickens. Avian Pathol 31, 399-406. Soriano, V.E., Tellez, G., Hargis, B.M., Newberry, L., Salgado-Miranda, C., Vazquez, J.C., 2004, Typing of Haemophilus paragallinarum strains by using enterobacterial repetitive intergenic consensus-based polymerase chain reaction. Avian Dis 48, 890-895. Stephens, C.P., Gibson, J.A., Blackall, P.J., 1990, Porcine pleuropneumonia in Australian pigs due to Actinobacillus pleuropneumoniae serovar 5. Aust Vet J 67, 462. Sun, H., Miao, D., Zhang, P., Gong, Y., Blackall, P.J., 2007, A comparison of a blocking ELISA and a haemagglutination inhibition assay for the detection of antibodies to Avibacterium (Haemophilus) paragallinarum in sera from artificially infected chickens. Biologicals 35, 317-320. Takagi, M., Hirayama, N., Makie, H., Ohta, S., 1991a, Production, characterization and protective effect of monoclonal antibodies to Haemophilus paragallinarum serotype A. Vet Microbiol 27, 327-338. Takagi, M., Ohmae, K., Hirayama, N., Ohta, S., 1991b, Expression of hemagglutinin of Haemophilus paragallinarum serotype A in Escherichia coli. J Vet Med Sci 53, 917-920. Thitisak, W., Janviriyasopak, O., Morris, R.S., Srihakim, S., van Kruedener, R., 1988, Causes of death found in an epidemiological study of native chickens in Thai villages. Acta Vet Scand Suppl 84, 200-202. Wambura, P.N., 2010, Preparation and use of autogenous vaccine from Avibacterium paragallinarum (strain Tan 1-05) in layer chickens. Trop Anim Health Prod 42, 483-486. Wang, H., Gao, Y., Gong, Y., Chen, X., Liu, C., Zhou, X., Blackall, P.J., Zhang, P., Yang, H., 2007, Identification and immunogenicity of an immunodominant mimotope of Avibacterium paragallinarum from a phage display peptide library. Vet Microbiol 119, 231-239. Welch, R.A., 1991, Pore-forming cytolysins of gram-negative bacteria. Mol Microbiol 5, 521-528. Welch, R.A., 2001, RTX toxin structure and function: a story of numerous anomalies and few analogies in toxin biology. Curr Top Microbiol Immunol 257, 85-111. Welch, R.A., Forestier, C., Lobo, A., Pellett, S., Thomas, W., Jr., Rowe, G., 1992, The synthesis and function of the Escherichia coli hemolysin and related RTX exotoxins. FEMS Microbiol Immunol 5, 29-36. Welchman Dde, B., King, S.A., Wragg, P., Wood, A.M., Irvine, R.M., Pepper, W.J., Dijkman, R., de Wit, J.J., 2010, Infectious coryza in chickens in Great Britain. Vet Rec 167, 912-913. Wu, J.R., Chen, P.Y., Shien, J.H., Shyu, C.L., Shieh, H.K., Chang, F., Chang, P.C., 2010, Analysis of the biosynthesis genes and chemical components of the capsule of Avibacterium paragallinarum. Vet Microbiol. Wu, J.R., Wu, Y.R., Shien, J.H., Hsu, Y.M., Chen, C.F., Shieh, H.K., Chang, P.C., 2011, Recombinant proteins containing the hypervariable region of the haemagglutinin protect chickens against challenge with Avibacterium paragallinarum. Vaccine 29, 660-667. Yamaguchi, T., Blackall, P.J., Takigami, S., Iritani, Y., Hayashi, Y., 1990, Pathogenicity and serovar-specific hemagglutinating antigens of Haemophilus paragallinarum serovar B strains. Avian Dis 34, 964-968. Yamaguchi, T., Kobayashi, M., Masaki, S., Iritani, Y., 1993, Isolation and characterization of a Haemophilus paragallinarum mutant that lacks a hemagglutinating antigen. Avian Dis 37, 970-976. Yamamoto, M., Saito, S., Sakurada, T., Yoshida, K., Kaise, K., Kaise, N., Yoshinaga, K., 1978, Correlation between per cent discharge of thiocyanate test and thyroid functions in patients with Hashimoto''s disease. Acta Endocrinol (Copenh) 87, 61-69. Yamamoto, R., 1997, Infectious coryza. Diseases of poultry, 10th ed, 8: 179-190. Zhang, P.J., Miao, M., Sun, H., Gong, Y., Blackall, P.J., 2003, Infectious coryza due to Haemophilus paragallinarum serovar B in China. Aust Vet J 81, 96-97. Zhao, Q., Sun, Y.N., Zhang, X.X., Kong, Y.B., Xie, Z.J., Zhu, Y.L., Zhou, E.M., Jiang, S.J., 2010, Evaluation of two experimental infection models for Avibacterium paragallinarum. Vet Microbiol 141, 68-72.
雞傳染性鼻炎 (infectious coryza) 係由Avibacterium paragallinarum感染雞隻造成上呼吸道疾病,常導致肉雞淘汰率增加與蛋雞產蛋性能下降,造成業界嚴重經濟損失。Av. paragallinarum目前可分為3種血清型,臺灣的田間感染以死菌疫苗免疫來控制,目前台灣地區每年所使用之疫苗數目皆在億劑以上。本研究可分為兩部分,第一部份是以新世代基因體定序技術,對血清型C型標準株H18株進行全基因體定序。以定序分析結果發現,在H18基因體序列中,存在多種與毒素合成相關的基因,其中RTX toxins (repeats in toxin) 合成相關基因為目前巴斯德桿菌科中最重要的毒力因子之一,可做為次單位疫苗成份。以PCR進行分析,發現不同之Av. paragallinarum菌株中,皆存在RTX toxins之基因。本研究為首度在Av. paragallinarum中找到RTX toxins基因之報導。H18株中所找到之 RTX 毒素操縱子 (operon) 基因群,命名為avxIABD,其中avxIA為主要之毒素基因,而avxIB及avxID則與毒素之分泌相關基因。AvxIA之基因全長約6.8Kb,可表現出大小為2286胺基酸之蛋白。以Pfam程式分析發現,AvxIA蛋白序列中存在12個Hemolysin type calcium-binding repeats與1個RTX C-terminal domain,此特性與其它RTX毒素類似。另一方面,AvxIA蛋白中也存在1個Peptidase_S8 domain,以及1個Proprotein convertase P-domain,此二結構區 (domains) 在其他RTX毒素中並未被發現過。為瞭解其中Peptidase_S8及Proprotein convertase P-domain之功能與抗原性,本研究將能表現含此二結構區之基因片段,選殖至原核表現系統中表現出85 kDa大小的重組蛋白,本研究以Western blot進行分析,結果顯示此蛋白可被標準株H18的高免血清辨認,未來將以此段蛋白研究生物功能與進行動物試驗,評估是否能做為疫苗抗原使用。本研究之第二部份,是針對先前已發表之Av. paragallinarum HA蛋白rF5片段進行分析,結果發現rF蛋白之主要抗原決定區,是位於271-462 bp位置,由此位置所表現之重組蛋白具有做為次單位疫苗之潛力。

Infectious coryza (IC) is an acute upper-respiratory disease caused by infection with Avibacterium paragallinarum. The disease is distributed worldwide and causes significant economic loss of poultry industry. In Taiwan, more than one hundred million doses of IC vaccine have been used every year to control the disease. The goal of this study is twofold. The first is to use the next generation sequencing technology to conduct sequence analyses of the complete genome of Av. paragallinarum strain H18, which is a serovar C reference strain. The result showed that H18 contains a number of genes involved in the biosynthesis of toxins. Among these toxins, the RTX toxin (repeat in toxin) is an important virulence factor of bacteria in the family Pasturellaceae. The RTX toxin has been used as a component of subunit vaccine. PCR analyses showed that the RTX gene is present in many strains of Av. paragallinarum. The gene encoding the RTX toxin is located at an operon named avxIABD, in which avxIA encodes the toxin while avxIB and avxID encode proteins required for secretion of the RTX toxin. The avxIA gene is 6861bp in size and has the potential to encode a protein of 2286 amino acid. Pfam analyses showed that AvxIA contains 12 copies of the hemolysin type calcium-binding domain and one copy of RTX C-terminal domain. These two domains have been found in other RTX toxins. However, AvxIA contains a peptidase_S8 domain and proprotein convertase P-domain, which are not found in other RTX toxins. To investigate the antigenicity and function of the peptidase_S8 domain and proprotein convertase P-domain, an 85 kDa recombinant protein containing these two domains were expressed in E. coli and purified with a Ni-affinity column. Western blot analyses showed that this recombinant protein reacted with hyper-immune serum against the whole-cell antigen of strain H18. The biological function of this recombinant and potential of using this protein as a vaccine antigen will be evaluated. The second part of this study is to investigate the antigenictity of the rF5 fragment from the HA protein of Av. paragallinarum. The result showed that the major antigenic determinant sites of rF5 is located at the 271-462 bp of the HA protein. Recombinant protein contain this region might have potential to be used as the antigens for subunit vaccines against Av. paragallinarum.
其他識別: U0005-1408201115564500
Appears in Collections:微生物暨公共衛生學研究所

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