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標題: 中部地區產蛋雞與帶殼蛋之沙門氏菌監測調查
Investigation of Salmonella in hens and shell-eggs in central Taiwan
作者: 蔡宇馨
Tsai, Yu-Shin
關鍵字: Salmonella;沙門氏菌;hen;egg;Antibiotic resistant;蛋雞;雞蛋;抗生素抗藥性
出版社: 獸醫公共衛生學研究所
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依據國家衛生研究院發表,台灣主要以Salmonella Typhimurium、Salmonella Enteritidis、Salmonella Stanley、Salmonella Agona、Salmonella Albany、Salmonella Schwarzengrund、Salmonella Newport、Salmonella Choleraesuis、Salmonella Derby及Salmonella Weltevreden等為人類常見感染的血清型。沙門氏菌症 (Salmonellosis) 爲重要的人畜共通傳染病,美國每年約有140萬人以上感染非傷寒性沙門氏菌症,造成800 – 4000 件死亡案例,以公共衛生的觀點,家禽感染沙門氏菌最易引起人們食物中毒的可能。本研究藉由調查中部地區蛋雞的沙門氏菌之陽性率,同時也對生鮮的雞蛋進行檢測,所得到的沙門氏菌菌株再進行抗生素的抗藥性試驗、血清分型及相關的危險因子分析,藉此提供未來擬訂沙門氏菌控制計畫的參考。採樣自蛋雞卵巢與十二指腸部份,總計採取990隻蛋雞,雞隻的陽性率為25.5 % (252/990);沙門氏菌的樣本總陽性檢出率為18.2 % (359/1970),卵巢的沙門氏菌陽性檢出率為蛋雞十二指腸沙門氏菌陽性檢出率的1.4倍 (95 % C.I 1.09 ~ 1.73)。夏、秋兩季溫度較高進而陽性率也跟著提高,秋季的沙門氏陽性檢出風險率是春季的2.8倍(95 % C.I 1.9 ~ 4.0)。雞蛋樣本部份並未檢測到任何沙門氏菌陽性。血清分型結果以Salmonella Enteritidis血清型為最多,佔總數的91.6 %(329/359),所有菌株的35.5 %(128/359)不具抗藥性,但全都是Salmonella Enteritidis血清型,其餘菌株主要是對Colistin及Tetracycline具有較高的抗藥性。在多變相邏輯式回歸分析中,在本次實驗得到在牧場中飼養的規模、消毒的次數、環境條件、周圍是否存在其他牧場、牧場的環境、飼主平均每日在牧場內工作的時數都會影響蛋雞牧場中沙門氏菌的陽性率。以上的研究資訊將有助於發展沙門氏菌的防治策略,以減低人類感染疾病的風險及提高農產品出口競爭力。

It is reported according to NHRI ( National Health Research Institutes) that the majority of human Salmonella cases in Taiwan are caused by serotypes of Salmonella Typhimurium、S. Enteritidis、S. Stanley、S. Agona、S. Albany、S. Schwarzengrund、S. Newport、S. Choleraesuis、S. Derby and S. Weltevreden. Salmonellosis is an important zoonosis worldwide. It was estimated that 1.4 million human cases with non-typhoidal Salmonella infections, and probably 800-4000 related deaths occur in the United States annually. In terms of public health, avian salmonellosis is a hazard to cause food poisoning cases in humans. The objectives of this study were 1) to investigate the positive rate of salmonellosis of hens and fresh eggs in central Taiwan; 2) to idethntify the antimicrobial resistance, serotyping, and associated risk factors of the Salmonella isolates; 3) to provide a protocol for control of Salmonellosis. A total of 990 samples were collected from chickens’ ovaries and duodenums. The Salmonella - positive rate of these chickens was 25.5%(252/990) and of all specimens was18.2% (359/1970). The percentage of Salmonella-positive specimens of ovaries was 1.4 times more than duodenums (95 % C.I 1.09 ~ 1.73). A significantly higher percentage of Salmonella-positive specimens was identified in summer and fall accompanying higher temperature. The Salmonella-positive specimens in fall is 2.8 times more than summer (95 % C.I 1.9 ~ 4.0). None samples of the eggs are salmonella-positive. In the event of serotyping, Salmonella Enteritidis was the most common serotype accounting for 91.6 %(329/359)of Salmonella isolates. And 35.5 %(128/359)of the isolates which were all Salmonella Enteritidis were not antimicrobial resistant . The other isolates are more resistant to Colistin and Tetracycline. Multivariate Statistical Analysis indicated that magnitude of rearing in farms, the frequencies of disinfection, the neighborhood, and the working hours of the farmer were all risk factors for Salmonella preavlance in hens farms. The above information will be helpful for the development of effective strategies for prevention and reducing the risk of human Salmonella infection to strength the competence for exporting agricultural products.
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