Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66365
DC FieldValueLanguage
dc.contributor葉光勝zh_TW
dc.contributor宣詩玲zh_TW
dc.contributor.advisor陳德勛zh_TW
dc.contributor.author翁嘉伶zh_TW
dc.contributor.authorLing, Weng Chiaen_US
dc.contributor.other中興大學zh_TW
dc.date2010zh_TW
dc.date.accessioned2014-06-09T09:31:31Z-
dc.date.available2014-06-09T09:31:31Z-
dc.identifierU0005-1906200915192600zh_TW
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dc.identifier.urihttp://hdl.handle.net/11455/66365-
dc.description.abstract沙門氏菌(Salmonella)為造成人類及動物胃腸炎的主要病原之一,沙門氏菌症(Salmonellosis)為世界性重要人畜共通傳染病,受感染之家禽及其相關製品為造成人類沙門氏菌症的主要來源。本研究藉由調查台灣地區傳統市場有色肉雞沙門氏菌感染比例、血清型別及抗藥性情形,並以聚合酶鏈鎖反應及脫氧核糖核酸基因定序分析比較不同物種所分離之沙門氏菌血清型別間是否有差異?以提供作為未來公共衛生之沙門氏菌監控計畫之參考。自2007年7月至2008年5月間,採集台灣南部及北部地區傳統市場有色肉雞之肝臟及膽囊進行分析,總採樣隻數為420隻雞,共分離到的174株沙門氏菌陽性分離株,其中,南部傳統市場共分離出58株,其陽性檢出率為13.81%;北部傳統市場共分離出116株,其陽性樣本檢出率為27.62%,結果發現北部傳統市場較南部傳統市場容易分離出沙門氏菌(p<0.05)。在膽囊檢體中共分離到100株陽性分離株,膽囊分離率為23.81%;在肝臟則分離到74株,肝臟分離率為17.62%,結果發現膽囊分離率顯著高於肝臟的分離率 (p<0.05)。檢出之常見血清型依序為 S. Albany(82.18%)、 S. Hadar (4.60%)、S. Enteritidis (4.60%)、 S. Thompson(2.87%)、 S. Schwarzengrund (2.30%)。在抗藥性比例中,一半以上分離株對抗菌劑如 Ampicillin、Colistin、Doxycycline、Florfenicol、Nalidixic acid及 Sulfamethoxazole- trimethoprim等具有抗藥性,且有多重抗藥性產生。將本實驗室自不同物種(人類、家禽及犬隻)分離之沙門氏菌進行DNA基因定序之分析,其結果顯示分離株之血清型為S. Albany、S. Enteritidis 及 S. Hadar 與基因庫之配對吻合度100%,而 S. Brandenburg 、S. Derby、 S. Thompson 分離株則分別顯示有不同的DNA及胺基酸序列差異點。以上研究資訊可提供作為沙門氏菌防治政策之參考,以降低沙門氏菌在公共衛生上之發生。zh_TW
dc.description.abstractSalmonella is the main pathogeny which causes human and animal gastroenteritis and also an important zoonosis. The infected poultry and related products are the major sources lead to Salmonellosis. This research attempt to figure out different serotype of Salmonella isolated from different species by investigating Salmonella infection rate, serotype, antimicrobial resistance, meanwhile, performing PCR (Polymerase Chain Reaction) test and DNA sequence analysis on colored meat chicken from Taiwan traditional market. The research result will be provided as a reference to Salmonella monitoring project in public health field. The research datas were collected from the liver and gall bladder of colored meat chicken from traditional market in southern and northern region during July 2007 to May 2008. The total samples are 420 chickens. A total of 174 Salmonella strains were isolated, 58 isolates as a rate of 13.81% from southern region; 116 isolates as a rate of 27.62% from northern region. It is significantly easier to isolate Salmonella from chicken in traditional market in northern region (p<0.05). From samples of gall bladder, 100 positive isolates were found which was as a rate of 23.81%. In liver, 74 positive isolates were found which was as a rate of 17.62%. The result shows that isolation rate from gall bladder is significantly higher than liver's (p<0.05). In addition, the most common serotypes are listed by their popularity: S. Albany(82.18%), S. Hadar (4.60%), S. Enteritidis(4.60%), S. Thompson(2.87%), S. Schwarzengrund(2.30%). In the antimicrobial resistance test, half of those isolates already produced antimicrobial resistance and even multidurg resistant Ampicillin, Colistin, Doxycycline, Florfenicol, Nalidixic acid and Sulfamethoxazole-trimethoprim. When comparing DNA sequence analysis on different serotype of isolated, they are perfectly matched with the GenBank of S. Albany, S. Enteritidis and S. Hadar. However, S. Brandenburg, S. Derby and S. Thompson isolates demonstrated different sequences in DNA sequence and amino acids. In conclusion, the overall results provide a data base as the reference for the Salmonella prevention policy in public healthen_US
dc.description.tableofcontents中文摘要 ………………………………………………………………………… i 英文摘要 ………………………………………………………………………… ii 目 次 ………………………………………………………………………………iii 表目次 …………………………………………………………………………… v 圖目次 …………………………………………………………………………… vi 附錄目次 …………………………………………………………………………vii 第一章 前 言 …………………………………………………………………… 1 第二章 文獻探討 ……………………………………………………………… 2 第一節 沙門氏菌簡介 ……………………………………………………… 2 一、歷史-命名 …………………………………………………………… 2 二、形態與特性 …………………………………………………………… 3 三、血清型分類 …………………………………………………………… 3 四、血清型別鑑定 ………………………………………………………… 4 五、沙門氏菌的書寫規則 ………………………………………………… 5 第二節 人類沙門氏菌症 …………………………………………………… 6 第三節 家禽沙門氏菌症 …………………………………………………… 6 第四節 沙門氏菌症流行病學 ……………………………………………… 7 第五節 沙門氏菌的抗藥性 ………………………………………………… 10 第六節 沙門氏菌鞭毛基因 ………………………………………………… 13 第七節 沙門氏菌分離與鑑定………………………………………………… 14 第八節 抗菌劑之規範 ……………………………………………………… 18 第九節 傳統市場活禽屠宰 ………………………………………………… 21 第十節 沙門氏菌之預防 …………………………………………………… 23 第三章 材料與方法 …………………………………………………………… 25 第一節 檢體採集 …………………………………………………………… 25 第二節 沙門氏菌分離與純化 ……………………………………………… 25 第三節 血清型鑑定 ………………………………………………………… 26 第四節 抗生素敏感性試驗 ………………………………………………… 28 第五節 DNA萃取 …………………………………………………………… 29 第六節 聚合酶鏈鎖反應……………………………………………………… 29 第七節 PCR產物回收 ……………………………………………………… 30 第八節 基因定序 …………………………………………………………… 31 第九節 基因定序分析 ……………………………………………………… 31 第十節 統計分析 …………………………………………………………… 31 第四章 實驗結果 ……………………………………………………………… 32 第一節 沙門氏菌分離率 …………………………………………………… 32 第二節 沙門氏菌血清型分類 ……………………………………………… 33 第三節 抗生素敏感性試驗 ………………………………………………… 34 第四節 聚合酶鏈鎖反應 …………………………………………………… 37 第五節 基因定序結果 ……………………………………………………… 38 第五章 討 論 …………………………………………………………………… 40 第一節 沙門氏菌陽性分離株檢出情形 …………………………………… 40 第二節 沙門氏菌血清型分佈情形 ………………………………………… 43 第三節 沙門氏菌分離株之抗藥性情形 …………………………………… 44 第四節 血清型之基因定序 ………………………………………………… 46 第六章 結 論 …………………………………………………………………… 47 參考文獻 ………………………………………………………………………… 79zh_TW
dc.language.isoen_USzh_TW
dc.publisher獸醫公共衛生學研究所zh_TW
dc.relation.urihttp://www.airitilibrary.com/Publication/alDetailedMesh1?DocID=U0005-1906200915192600en_US
dc.subjectColored Meat Chickenen_US
dc.subject有色肉雞zh_TW
dc.subjectSalmonellaen_US
dc.subjectSerotypeen_US
dc.subjectAntimicrobial Resistanceen_US
dc.subject沙門氏菌zh_TW
dc.subject血清型zh_TW
dc.subject抗藥性zh_TW
dc.title台灣傳統市場有色肉雞沙門氏菌血清型及抗藥性之研究zh_TW
dc.titleThe Research for Serotype and Antimicrobial Resistance of Colored Meat Chicken Salmonella in Taiwan Traditional Marketen_US
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeThesis and Dissertation-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
Appears in Collections:微生物暨公共衛生學研究所
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