Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66390
標題: 幾丁聚醣-奈米金複合材料之微溝槽人工周邊神經導管之修復功能
Sciatic Nerve Repair by Microgrooved Nerve Conduits Made of Chitosan-gold Nanocomposites
作者: 任睿麒
Jen, Jui-Chi
關鍵字: Microgroove;微溝槽;gold nanocomposite;chitosan;PLA;nerve conduit;nerve repair;奈米金;幾丁聚醣;聚乳酸;神經導管;神經修復
出版社: 獸醫微生物學研究所
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摘要: 
外傷病人中有2.8%受到周邊神經的傷害影響。神經自體移植是周邊神經修復的標準方法,但是如果神經的缺損部份太大而無法修復時,就需要人工神經導管。神經導管能提供修復中的新生神經方向性和支持性,促使組織修復。在人工神經導管植入排序之細胞更可加速周邊神經修復。本研究首先製備幾丁聚醣-奈米金複合材料含微溝槽膜,發現細胞生長情形在幾丁聚醣-奈米金複合材料含微溝槽膜上明顯優於單純幾丁聚醣微溝槽膜。將材料製成神經導管後,以聚乳酸包覆幾丁聚醣神經導管以加強機械強度,並先行於導管中植入小鼠神經幹細胞,進行大鼠坐骨神經缺損動物實驗,六週後,發現幾丁聚醣-奈米金複合材料含微溝槽導管可加速坐骨神經10 mm缺口之修復連接,並且新生的神經組織中已有新生的髓鞘形成。

Peripheral nerve injury affects up to 2.8% of trauma patients. The gold standard for repairing peripheral nerve injury gaps is the nerve autograft. However, if the nerve injury gap is too long to repair, an artificial nerve conduit is needed. The nerve conduit can provide the structural support and facilitate tissue repair. The conduits with microgrooved inner lumen and seeded with aligned Schwann cells were known to accelerate the peripheral nerve repair. In this study, microgrooved films made of chitosan-gold nanocomposite were found to align cells better than the microgrooved pristine chitosan. The microgrooved conduits made by the chitosan-gold nanocomposite films seeded with mouse neural stem cells were implanted into the 10 mm rat sciatic nerve defects. After 6 weeks, the repair in the nanocomposite microgrooved conduits was superior to that in the chitosan microgrooved conduits or the conduits without neural stem cells. Myelination was found inside the nanocomposite microgrooved conduits by histological examination.
URI: http://hdl.handle.net/11455/66390
其他識別: U0005-3007200619485300
Appears in Collections:微生物暨公共衛生學研究所

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