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Expression of Capsid Proteins and Replication Proteins of Waterfowl Circoviruses in Baculovirus System and Application in Serological Survey
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水禽環狀病毒為新興的水禽病原，可分為鵝環狀病毒與鴨環狀病毒，病毒感染後易造成免疫抑制，因而增加其它疾病的罹患率。先前本研究室曾使用E. coli表現系統，表現水禽類環狀病毒之複製蛋白(Rep)與外殼蛋白(Cap)，以作為診斷環狀病毒抗體之抗原。結果發現E. coli系統雖可順利表現病毒之Rep蛋白，但卻無法順利表現病毒之Cap蛋白。本研究之目的是以桿狀病毒系統，表現鴨鵝環狀病毒之Rep蛋白與Cap蛋白，並以此二表現蛋白為抗原，進行Western blot分析，以檢測台灣田間水禽環狀病毒之抗體。結果發現桿狀病毒系統可成功表現鴨鵝環狀病毒之全長Rep蛋白與Cap蛋白，進一步以此Cap及Rep蛋白為抗原，進行Western blot分析，以檢測鴨鵝環狀病毒之抗體，結果發現所檢測到之環狀病毒抗體力價皆不高，顯示水禽環狀病毒感染後可能無法產生高力價抗體。這是第一篇以桿狀病毒系統，成功表現出水禽環狀病毒之全長複製蛋白(Rep)與外殼蛋白(Cap)之報告。
Waterfowl circoviruses are emerging diseases of waterfowls and these viruses could be divided into goose circovirus (GoCV) and duck circoviruses (DuCV). The infection of waterfowl circoviruses might lead to immuno suppression and increase the incidence of other diseases. This laboratory previously used the E. coli expression system to express the replication protein (Rep) and capsid protein (Cap) of GoCV and DuCV but found that the only the Rep protein but not the Cap protein could be expressed. The goal of this study is to use the baculovirus expression system to express the Rep and Cap proteins of GoCV and DuCV and to use these proteins as antigens to detect the antibodies against GoCV and DuCV in geese and ducks. The result shows that Rep and Cap proteins of GoCV and DuCV could all be successfully expressed in baculovirus expression system. Moreover, Western blot assays using these proteins as the antigens showed that the antibody titers against GoCV and DuCV were low in sera collected from geese and ducks in the field. This result suggests that the infection of GoCV and DuCV might produce only a low level of antibody response. This is the first report on the expression of the full-length Rep and Cap proteins of GoCV and DuCV.
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