Please use this identifier to cite or link to this item:
標題: 台灣中部地區家禽里奧病毒之田間調查、 病毒分離及基因型分析
Field Investigation, Virus Isolation and Genotype Analysis of Avian Reovirus in Central Part of Taiwan
作者: 余忠杰
Yu, Chung-Chieh
關鍵字: avian reovirus;家禽里奧病毒
出版社: 獸醫微生物學研究所
引用: 參考文獻 1. 林雅玲。2004。碩士論文。應用競爭性酵素接合免疫吸附法檢測雞隻家禽里奧病毒抗體。國立中興大學獸醫學研究所。台灣省台中市。中華民國。 2. Barta V, Springer W T, Miller D L: A comparison of avian and mammalian cell cultures for the propagation of avian reovirus WVU 2937.Avian Dis 28: 216-223, 1984. 3. Benavente Javier, Martínez-Costas Jose: Avian reovirus: structure and biology. Virus Res 123: 105-119, 2007. 4. Benavente J, Shatkin A J: Avian reovirus mRNAs are nonfunctional in infected mouse cells: translational basis for virus host-range restriction. Proc Natl Acad Sci 85: 4257-4261, 1988. 5. Bodelon G, Labrada L, Martinez-Costas J, Benavente J: The avian reovirus genome segment S1 is a functionally tricistronic gene that expresses one structural and two nonstructural proteins in infected cells. Virology 290: 181-191, 2001. 6. Bulow V V, Klasen A: Effect of avian reovirus on cultured chicken bone-marrow-derived macrophages. Avian Dis 12: 179-198, 1983. 7. Day Michael J, Pantin-Jackwood Mary J, Spackman Erica: Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reoviruses. Virus Genes 35: 235-242, 2007. 8. Deshmukh D R, Dutta S K, Pomeroy B S: Avian reoviruses. V. Studies of ultrastructural morphology by electron microscopy. Avian Dis 15: 588-595, 1971. 9. Deshmukh D R, Pomeroy B S: Avian reoviruses. Ⅲ Infectivity and egg transmission. Avian Dis 13: 427-439, 1969. 10. Fahey J E, Crawley J F: Studies on chronic respiratory disease of chickens. II. Isolation of a virus. Can J Comp Med 18: 13-21, 1954. 11. Giambrone J J, Solano W: Serologic comparison of avian reovirus isolates using virus neutralization and an enzyme-linked immunosorbent assay. Avian Dis 32: 678-680, 1988. 12. Guneratne J R M, Jones R C, Georgiou K: Some observations on the isolation and cultivation of avian reoviruses. Avian Pathol 11: 453-462, 1982. 13. Wood G W, Nicholas R A, Hebert C N, Thornton D H: Serological comparisons of avian reoviruses. J Comp Pathol 90: 29-38, 1980. 14. Glass, S. E., S. A. Naqi, C. F. Hall, and K. M. Kerr. 1973. Isolation and characterization of a virus associated with arthritis of chickens. Avian Dis. 17:415-424. 15. Gouvea V S, Schnitzer T J: Polymorphism of the migration of double-stranded RNA genome segments of avian reoviruses. J Virol 43: 465-471, 1982. 16. Grande A, Benavente J: Optimal conditions for the growth, purification and storage of the avian reovirus S1133. J Virol Methods 85: 43-54, 2000. 17. Johnson D C: Diagnosis, pathology, and etiology of tenosynovitis in broilers and broiler breeders. Avian Dis 16: 1067-1072, 1972. 18. Jones R C, Guneratne J R M: The pathogenicity of some avian reoviruses with particular reference to tenosynovitis. Avian Pathol 13: 173-189, 1984. 19. Jones R C, Jordan T W, Lioupis S: Characteristics of reovirus isolated from ruptured gastrocnemius tendons of chickens. Vet Rec 96: 153-154, 1975. 20. Kawamura H, Shimizu F, Maeda M, Tsubahara H: Avian reovirus: its properties and serological classificaton. Natl Inst Anim Health Q 5: 115-124, 1965. 21. Lee L F, Nazerian K, Burmester B R: Characterization of avian reovirus 24. Avian Dis 17: 559-567, 1973. 22. Lee L H, Wang Y H, Shien J H: Serological chcracterization of reoviruses isolated from avian species in Taiwan. J Chin Vet Sci 18: 69-77, 1992. 23. Liu H J, Giambrone J J: In situ detection of reovirus in formalin-fixed, paraffin-embedded chicken tissues using a digoxigenin-labeled cDNA probe. Avian Dis 41: 447-451, 1997. 24. Mallo M, Martinez-Costas J, Benavente J: Avian reovirus S1133 can replicate in mouse L cells: effect of pH and cell attachment status on viral infection. J Virol 65: 5499-5505, 1991. 25. Martínez-Costas J, Grande A, Varela R, García-Martínez C, Benavente J: Protein architecture of avian reovirus S1133 and identification of the cell attachment protein. J Virol 71: 59-64. 1997. 26. Menendez N A, Calnek B W, Cowen B S: Experimental egg-transmission of avian reovirus. Avian Dis 19: 104-111, 1975. 27. Metcalf P, Cyrklaff M , Adrian M: The three-dimensional structure of reovirus obtained by cryo-electron microscopy. EMBO J 10: 3129-3136, 1991. 28. Nick H, Cursiefen D, Becht H: Structural and growth characteristics of two avian reoviruses. Arch Virol 48: 261-269, 1975. 29. Ni Y, Kemp M C: A comparative study of avian reovirus pathogenicity: virus spread and replication and induction of lesions. Avian Dis 39: 554-566, 1995. 30. Ni Y, Ramig R F, Kemp M C: Identification of proteins encoded by avian reoviruses and evidence for post-translational modification. Virology 193: 466-469, 1993. 31. Olson N O, Kerr K M: Some characteristics of an avian arthritis viral agent. Avian Dis 10: 470-476, 1966. 32. Olson N O, Kerr K M: The duration and distribution of synovitis-producing agent in chickens. Avian Dis 11: 578-585, 1967. 33. Page R K, Fletcher Jr O J, Villegas P: Infectious tenosynovitis in young turkeys. Avian Dis 26: 924-927, 1982. 34. Parker J S L, Broering T J, Kim J, Higgins D E, Nibert M L: Reovirus core protein μ2 determines the filamentous morphology of viral inclusion bodies by interacting with and stabilizing microtubules. J Virol 76: 4483-4496, 2002. 35. Robertson M D, Wilcox G E: Avian reovirus. Veterinary Bulletin 56: 155-174, 1986.Bains B S, MacKenzie M, Spradbrow P B: Reovirus-associated mortality in broiler chickens. Avian Dis 18: 472-476, 1974. 36. Sahu S P, Olson N O: Comparison of the characteristics of avian reoviruses isolated from the digestive and respiratory tract, with viruses isolated from the synovia. Am J Vet Res 36: 847-850, 1975. 37. Shapouri M R, Arella M, Silim A: Evidence for the multimeric nature and cell binding ability of avian reovirus sigma 3 protein. J Gen Virol 77: 1203-1210, 1996. 38. Shapouri M R, Reddy S Silim K A: Interaction of avian reovirus with chicken lymphoblastoid cell lines. Avian Pathol 23: 287-296, 1994. 39. Spandidos D A, Graham A F: Physical and chemical characterization of an avian reovirus. J Virol 19: 968-976, 1976. 40. Spackman E, Pantin-Jackwood M J, Day J M, Sellers H S: The pathogenesis of turkey origin reoviruses in turkeys and chickens. Avian Path 34: 291-296, 2005. 41. Sterner F J, Rosenberger Margolin J K A, Ruff M D: In vitro and in vivo characterization of avian reoviruses. II. Clinical evaluation of chickens infected with two avian reovirus pathotypes. Avian Dis 33: 545-554, 1989. 42. Takehara K, Kimura Y, Tanaka Y, Yoshimura M: Preparation and characterization of monoclonal antibodies against an avian reovirus. Avian Dis 31: 730-734, 1987. 43. Taylor D L, Olson N O, Burrell R G: Adaptation of an avian arthritis viral agent to tissue culture. Avian Dis 10: 462-470, 1966. 44. Tourís-Otero F, Cortez-San Martín M, Martínez-Costas J, Benavente J: Avian reovirus morphogenesis occurs within viral factories and begins with the selective recruitment of σNS and λA to μNS inclusions, J Mol Biol 341: 361-374, 2004. 45. Tourís-Otero F, Martínez-Costas J, Vakharia V N, Benavente J: Avian reovirus nonstructural protein μNS forms viroplasm-like inclusions and recruits σNS to these structures. Virology 319: 94-106, 2004. 46. Tourís-Otero F, Martínez-Costas J, Vakharia V N, Benavente J: Characterization of the nucleic acid-binding activity of the avian reovirus nonstructural protein σNS. J Gen Virol 86: 1159-1169, 2005. 47. Van der Heide L, Kalbac M: Infectious tenosynovitis (viral arthritis): characterization of a Connecticut viral isolant as a reovirus and evidence of viral egg transmission by reovirus-infected broiler breeders. Avian Dis 19: 683-688, 1975. 48. Van der Heide L, Lutticken D, Horzinek M: Isolation of avian reovirus as a possible etiologic agent of osteoporosis ("brittle bone disease" "femoral head necrosis") in broiler chickens. Avian Dis 25: 847-856, 1981. 49. Varela R, Benavente J: Protein coding assignment of avian reovirus strain S1133. J Virol 68: 6775-6777, 1994. 50. Varela R, Martínez-Costas J, Mallo M, Benavente J: Intracellular posttranslational modifications of S1133 avian reovirus proteins, J Virol 70: 2974-2981, 1996, 51. Walker E R, Friedman M H, Olson N O: Electron microscopic study of an avian reovirus that causes arthritis. J Ultrastruct Res 41: 67-79, 1972. 52. Wickramasinghe R, Meanger J, Enriquez C E, Wilcox G E: Avian reovirus proteins associated with neutralization of virus infectivity. Virology 194: 688-696, 1993. 53. Wilcox G E, Robertson M D, Lines A D: Adaption and characteristics of replication of a strain of avian reovirus in vero cells. Avian Pathol 14: 321-328, 1985. 54. Wu W Y, Shien J H, Lee L H, Shieh H K: Analysis of the double-stranded RNA genome segments among avian reovirus field isolates. J Virol Methods 48: 119-122, 1994. 55. Yin H S, Lee L H: Identification and characterization of RNA-binding activities of avian reovirus non-structural protein σNS. J Gen Virol 79: 1411-1413, 1998.
家禽里奧病毒 (ARV, Avian reovirus) 為一種會造成家禽產業嚴重損失的重要致病原,其乃少數能引起細胞與細胞間融合的病毒,通常在患病雞隻會出現病毒性關節炎、營養吸收不良症及慢性呼吸道疾病。本實驗利用針對ARV S4基因之特異性引子對,將來自台中、南投、彰化及雲林等台灣中部縣市之雞隻糞便及內臟檢體,經製備乳劑後,接種於雞胚纖維芽母細胞(CEF, Chicken embryo fibroblast)內進行病毒分離,再以反轉錄聚合酶鏈鎖反應(RT-PCR, Reverse transcription-polymerase chain reaction)偵測後,將定序結果與NCBI之基因庫進行比對,確定是否為家禽里奧病毒;同時以針對變異性最高的S1基因之特異性引子對,進一步將前述確定有ARV之檢體進行增幅,並分析其基因型別。本實驗成功地由雲林台中等地分離到五株家禽里奧病毒,將其分別編號為1~ 5,經由比對後確認這些病毒分離株之S1基因序列分別與分離自德國的GEL13b98M株、來自美國的NC/SEP-R61/03株及138株最為相近。由親源樹分析中可以得知這五株分離株與台灣所使用之疫苗株(S1133等)及分離自台灣(1970-1992)的分離株分別屬於不同的群落(lineage)。

Avian reovirues are important pathogens of poultry industry, because they can cause considerable losses of the farming economy. There are very few viruses have the ability to cause cell-cell fusion, and ARV is one of them. ARV usually induces arthritis, malabsorption syndrome and chronic respiratory disease. The feces and organs of chickens were collected from Taichung, Nantou, Changhua and Yunlin. The samples described above were inoculated into CEF (chicken embryo fibroblast) to isolate the virus, and detected by RT-PCR method. Sequencing results were compared with the data saved in Genebank to confirm the ARVs existing. In the same time, we used a pair of primers specific to ARV S1 gene to amplify the target sequences, and followed by genotype analysis. This study successful isolated five field ARVs, temporally assigned numbers from 1 to 5. The S1 gene of the five field strains have the closest relation with GEL13b98M (isolated from Germany), NC/SEP-R61/03 and 138 (isolated from US). The five field strains isolated from this study, ARV vaccine strains and the ARV strains isolated from Taiwan in 1970~1992 could be classified into different lineages.
其他識別: U0005-3107200716472200
Appears in Collections:微生物暨公共衛生學研究所

Show full item record
TAIR Related Article

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.