Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66414
標題: 台灣中部地區家禽里奧病毒之田間調查、 病毒分離及基因型分析
Field Investigation, Virus Isolation and Genotype Analysis of Avian Reovirus in Central Part of Taiwan
作者: 余忠杰
Yu, Chung-Chieh
關鍵字: avian reovirus;家禽里奧病毒
出版社: 獸醫微生物學研究所
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摘要: 
家禽里奧病毒 (ARV, Avian reovirus) 為一種會造成家禽產業嚴重損失的重要致病原,其乃少數能引起細胞與細胞間融合的病毒,通常在患病雞隻會出現病毒性關節炎、營養吸收不良症及慢性呼吸道疾病。本實驗利用針對ARV S4基因之特異性引子對,將來自台中、南投、彰化及雲林等台灣中部縣市之雞隻糞便及內臟檢體,經製備乳劑後,接種於雞胚纖維芽母細胞(CEF, Chicken embryo fibroblast)內進行病毒分離,再以反轉錄聚合酶鏈鎖反應(RT-PCR, Reverse transcription-polymerase chain reaction)偵測後,將定序結果與NCBI之基因庫進行比對,確定是否為家禽里奧病毒;同時以針對變異性最高的S1基因之特異性引子對,進一步將前述確定有ARV之檢體進行增幅,並分析其基因型別。本實驗成功地由雲林台中等地分離到五株家禽里奧病毒,將其分別編號為1~ 5,經由比對後確認這些病毒分離株之S1基因序列分別與分離自德國的GEL13b98M株、來自美國的NC/SEP-R61/03株及138株最為相近。由親源樹分析中可以得知這五株分離株與台灣所使用之疫苗株(S1133等)及分離自台灣(1970-1992)的分離株分別屬於不同的群落(lineage)。

Avian reovirues are important pathogens of poultry industry, because they can cause considerable losses of the farming economy. There are very few viruses have the ability to cause cell-cell fusion, and ARV is one of them. ARV usually induces arthritis, malabsorption syndrome and chronic respiratory disease. The feces and organs of chickens were collected from Taichung, Nantou, Changhua and Yunlin. The samples described above were inoculated into CEF (chicken embryo fibroblast) to isolate the virus, and detected by RT-PCR method. Sequencing results were compared with the data saved in Genebank to confirm the ARVs existing. In the same time, we used a pair of primers specific to ARV S1 gene to amplify the target sequences, and followed by genotype analysis. This study successful isolated five field ARVs, temporally assigned numbers from 1 to 5. The S1 gene of the five field strains have the closest relation with GEL13b98M (isolated from Germany), NC/SEP-R61/03 and 138 (isolated from US). The five field strains isolated from this study, ARV vaccine strains and the ARV strains isolated from Taiwan in 1970~1992 could be classified into different lineages.
URI: http://hdl.handle.net/11455/66414
其他識別: U0005-3107200716472200
Appears in Collections:微生物暨公共衛生學研究所

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