Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/66469
標題: 台灣豬隻困難腸梭菌盛行率與流行病學之研究
Prevalence and Epidemiology of Clostridium difficile Isolated from Swine in Taiwan
作者: 劉宜芬
Liu, Yi-Fen
關鍵字: 困難腸梭菌;Clostridium difficile;盛行率;流行病學;毒素基因型;人畜共通;prevalence;epidemiology;toxigenic type;zoonosis
出版社: 微生物暨公共衛生學研究所
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摘要: 
由於高毒力菌株的發現,近年來困難腸梭菌(Clostridium difficile)逐漸為人們所重視。困難腸梭菌主要會引起人類以及家畜動物產生下痢等之腸胃道症狀。然而,目前為止台灣仍缺乏動物中引發困難腸梭菌感染症等等的相關研究報告。因此,為了解困難腸梭菌在台灣之流行病學分布情形,本次共自12場豬場採樣187份樣本進行細菌分離等實驗,並收集了人類臨床菌株,以期能了解人類與動物菌株之差異之處。研究結果顯示,自豬隻檢體中分離出困難腸梭菌的總盛行率為52.94% (99/187),其中以母豬(57.53%, 42/73)以及哺乳豬(56.57%, 56/99)為最高。此外,研究中也發現,豬隻年齡以及豬場皆為影響困難腸梭菌分離率之因子。總體而言,本次實驗中豬隻菌株毒力高於人類菌株。藥物敏感性試驗部分,豬隻菌株對於penicillin G之抗藥性遠高於人類來源菌株,代表抗藥性問題可能普遍存在豬場之中。最後,根據PCR核酸分型的結果發現,豬隻採樣樣本若來自於同一地點則菌株之親源性也較高,且經本次研究結果認為,人與動物之間的傳播能力相當有限。雖然目前尚未有困難腸梭菌造成台灣畜牧業重大經濟損失的相關報告出現,但往後對於困難腸梭菌相關性疾病的防治仍然非常重要。本篇研究為第一篇在台灣研究豬隻困難腸梭菌流行病學之報告。

Clostridium difficile, which has been regarded as an important pathogen that causes diarrhea in human and productive animals, has received increased interest due to the observation of hypervirulent strain. However, there are little investigations published about C. difficile in animals in Taiwan. To understand the epidemiology, a total of 187 samples from 12 pig farms in Taiwan were collected for isolation and 19 human strains from hospitals were used to investigate the difference between human and animals. The overall prevalence of C. difficile in pigs were 52.94% (99/187) with sows (57.53%, 42/73) and nursing pigs (56.57%, 56/99) the highest. Besides age, farms were also the important influence factors to affect C. difficile colonization. Overall, pig strains isolated in this study were more virulent than human strains after detecting their toxin gene patterns (tcdA/tcdB genes and cdtA/cdtB genes). The results of antimicrobial susceptibility tests also showed that pig strains were much more resistant to penicillin G than human strains, which might indicate the problem of antibiotic resistance. Finally, according to the results of PCR ribotyping, samples from the same farm might lead to higher similarity than others, moreover, due to the electrophoresis images and the phylogenetic trees, the results tend to have limited transmission possibilities between human and animals. Although there are few reports mentioned about the great econocmic loss caused from C. difficile infection in Taiwan, the surveillance of disease outbreak is still important. This study is also the first C. difficile report in swine populations in Taiwan.
URI: http://hdl.handle.net/11455/66469
其他識別: U0005-1106201316031000
Appears in Collections:微生物暨公共衛生學研究所

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