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標題: Evaluation and application of conducting polymer entrapment on quartz crystal microbalance in flow injection immunoassay
作者: Liu, Y.C.
Wang, C.M.
Hsiung, K.P.
Huang, C.J.
關鍵字: quartz crystal microbalance;flow-injection-analysis;conducting polymer;entrapment;pseudorabies virus;point-of-care testing;glucose-oxidase;immobilization;biosensors;immunosensors;polypyrrole;disease;virus;films
Project: Biosensors & Bioelectronics
期刊/報告no:: Biosensors & Bioelectronics, Volume 18, Issue 7, Page(s) 937-942.
An immunosensor employing conducting polymer entrapment (CPE) method to immobilize immuno-protein on the quartz crystal microbalance (QCM) for clinical flow-injection-analysis (FIA) purpose was exploited. By comparing the CPE approach and the conventional physical immobilization (PI) method, a frequency shift of the former was 47% higher than that of the latter when measuring at 0.5 mg/ml human serum albumin antibody concentration. This implied that CPE was a feasible approach for developing QCM-FIA process. An immunoassay of anti-pseudorabies virus antibody in mouse sera further exemplified its practical potential in diagnostic implication. (C) 2002 Elsevier Science B.V. All rights reserved.
ISSN: 0956-5663
DOI: 10.1016/s0956-5663(02)00208-7
Appears in Collections:微生物暨公共衛生學研究所

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