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標題: Recombinant proteins containing the hypervariable region of the haemagglutinin protect chickens against challenge with Avibacterium paragallinarum
作者: Wu, J.R.
Wu, Y.R.
Shien, J.H.
Hsu, Y.M.
Chen, C.F.
Shieh, H.K.
Chang, P.C.
關鍵字: Avibacterium paragallinarum;Haemagglutinin;HA;Vaccine;pathogen moraxella-catarrhalis;haemophilus-paragallinarum;hemophilus-paragallinarum;pasteurella-multocida;serological;classification;filamentous hemagglutinin;yersinia-enterocolitica;infectious coryza;adhesin yada;binding
Project: Vaccine
期刊/報告no:: Vaccine, Volume 29, Issue 4, Page(s) 660-667.
The haemagglutinin (HA) protein plays a key role in the immunogenicity and pathogenicity of Avibacterium paragallinarum, but the domain organization and antigenicity exhibited by different domains of this protein remain unknown. This study reports the presence of a hypervariable region in the HA proteins of strains of serovars A and C of A. paragallinarum. This hypervariable region is located approximately at residues 1100-1600 of the HA protein. The sequence identity found in this hypervariable region was only 18.1%, whereas those upstream and downstream of this region were 83.8 and 97.8%, respectively. Western blot analyses using antisera against the whole-cell antigens of A. paragallinarum showed that the hypervariable region was more antigenic than other regions of the HA protein. Moreover, the antigenicity of the hypervariable region was serovar-specific. Chickens immunized with recombinant proteins that contained the hypervariable region were protected (83-100% protection rate) against challenge infection with A. paragallinarum of the homologous serovar. These results suggest that recombinant proteins containing the hypervariable region may be useful antigens for use in the development of a vaccine against A. paragallinarum. (C) 2010 Elsevier Ltd. All rights reserved.
ISSN: 0264-410X
DOI: 10.1016/j.vaccine.2010.11.040
Appears in Collections:微生物暨公共衛生學研究所

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